Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine, Mibu, Tochigi 321-0293, Japan
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine, Mibu, Tochigi 321-0293, Japan
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine, Mibu, Tochigi 321-0293, Japan
雑誌名
Dokkyo Journal of Medical Sciences
巻
48
号
2
ページ
81 - 86
発行年
2021-07-25
要旨
Purpose：The effect of 5-HT2B receptor-selective agonist BW723C86 was investigated on the outflow of 5-hydroxytryptamine（5-HT）from isolated muscle layer-free mucosal preparations of guinea-pig colon.
Methods：The mucosal preparations were incubated in vitro and the outflow of 5-HT from these preparations was determined by high-performance liquid chromatography with electrochemical detection.
Results：BW723C86（1-10 μM）produced a sustained increase in the outflow of 5-HT from the mucosal preparations. The BW723C86-evoked 5-HT outflow was inhibited by the two different 5-HT2B receptor antagonists, olanzapine（100 nM）and RS127445（100 nM）. The neuropeptide Y1 receptor antagonist BIBO3304（300 nM）markedly inhibited the BW723C86-evoked 5-HT outflow.
Conclusion：We found that 5-HT2B receptor-triggered 5-HT release from guinea-pig colonic mucosa is mediated by the activation of 5-HT2B receptors located at endocrine cells and that the 5-HT2B receptortriggered 5-HT release is mediated by endogenously released peptide YY, acting via Y1 receptors. Given that both 5-HT and peptide YY are well-known mediators of nausea and vomiting, the 5-HT2B receptors located at endocrine cells may play a role in the generation of nausea and vomiting.
5-HT2B Receptors-triggered Serotonin Release from Guinea-pig Isolated Colonic Mucosa：a Role of Endogenous Peptide YY
DJMS-48-2-Kojima-本文
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