2026-03-15T18:03:37Z https://dmu.repo.nii.ac.jp/oai

oai:dmu.repo.nii.ac.jp:00000997 2023-06-20T16:03:25Z 81:61

Diagnostic Validity of DNMT-1 and 3b Immunoreactivity in Non-neoplastic Epithelium of UC Patients with and Without Neoplasia Diagnostic Validity of DNMT-1 and 3b Immunoreactivity in Non-neoplastic Epithelium of UC Patients with and Without Neoplasia Tanaka, Hiroyuki Ichikawa, Kazuhito Fujimori, Takahiro Ohtake, Shinichiro Yasuda, Yoshikazu DNA methyltransferase surveillance ulcerative colitis-associated neoplasia It is important to improve the efficacy of surveillance in UC patients with neoplasia.In the present study, we assessed the cut-off value of expression of DNMT-1 and DNMT-3b expression in the non-neoplastic rectal epithelium of patients with long-standing and extensive UC. Sixty patients with long-standing and extensive UC participated in this study( 30 with colorectal neoplasia and 30 without). Immunohistochemical analysis was performed to determine the expression of DNMT-1 and 3b in non-neoplasticrectal epithelium of UC patients without neoplasia, and in non-neoplastic rectal epithelium of UC patients with neoplasia. The level of immunoreactive DNMT-1 and DNMT-3b expression was determined as the percentage of positive cells relative to the total number of cells counted under high power magnification.DNMT-1 and 3b expression in non-neoplastic rectal epithelium of UC patients with neoplasia (0.57, range0.53-0.63)(0.32, range 0.18-0.67) was higher than in the non-neoplastic epithelium of UC patients without neoplasia (0.41, range 0.25-0.54, P=.001)(0.0, range 0.0-0.13, P<.001). ROC curve analysis confirmed0.53 and 0.07 as the best DNMT-1 and DNMT-3b cut-off values for identifying individuals at increased risk of neoplasia( area under the curve=0.798 and 0.842, respectively). The cut-off value for DNMT-1 andDNMT-3b expression in non-neoplastic rectal epithelium is therefore an efficient predictor for the increased risk of UC-associated neoplasia. Original 2012-03-25 eng journal article https://dmu.repo.nii.ac.jp/records/997 AA00629581 03855023 Dokkyo journal of medical sciences 39 1 29 35 https://dmu.repo.nii.ac.jp/record/997/files/KJ00007845699.pdf application/pdf 192.0 kB 2017-05-24