2026-03-12T23:13:51Z https://dmu.repo.nii.ac.jp/oai

oai:dmu.repo.nii.ac.jp:00001492 2023-06-20T15:45:17Z 81:87

Supplementation of Cryomedium with Catalase and N-acetylcysteine Improves Human Sperm Post-thaw Motility and DNA Integrity Kobori, Yoshitomo Okada, Hiroshi Niederberger, Craig Antioxidant DNA damage Sperm cryopreservation Purpose：Reactive oxygen species have been suggested as a major contributing factor to cryodamage of the spermatozoa. Accordingly, antioxidant supplementation has been used to yield significantly improved quality of frozen sperm post-thaw. We sought to investigate outcomes with a combination of antioxidants catalase and N-acetylcysteine respectively. Methods：The cryomedium supplemented with 200U/ml catalase, 5 mM N-acetylcysteine and their combination was used. Semen samples were collected from normozoospermic men （n＝20）and aliquots frozen in each dose of antioxidant tested. Post-thaw semen analysis by computer aided sperm analysis was performed. In addition, sperm viability （Eosin-Y staining） and sperm DNA fragmentation（TUNEL assay）were quantitated. Results：Combined catalase and N-acetylcysteine supplementation during cryopreservation resulted in significantly improved post-thaw recovery of total motility（from 39.4±15.8％ to 49.2±16.1％） and percentage of sperm vitality （from 46.2±13.3％ to 53.2±11.8％）（mean±SD, p＜0.05）. Catalase improved post-thaw DNA integrity analysis for doublestranded breaks using TUNEL assay. Conclusions：These results indicated that the combination of intracellular and extracellular antioxidants resulted in the pronounced effect in improving post-thaw quality of human spermatozoa. 獨協医学会 2017-07-25 eng journal article https://dmu.repo.nii.ac.jp/records/1492 AA00629581 03855023 Dokkyo Journal of Medical Sciences 44 2 135 140 https://dmu.repo.nii.ac.jp/record/1492/files/DJMS-44-2-2.pdf application/pdf 75.2 kB 2018-07-10