@article{oai:dmu.repo.nii.ac.jp:00001174,
 author = {根本, 興平 and Nemoto, Kohei},
 issue = {1},
 journal = {Dokkyo journal of medical sciences},
 month = {Mar},
 note = {A 型ボツリヌス毒素(BoNT/A)の神経障害性疼痛に対する鎮痛機序を明らかにするために,マウスの坐骨神経部分結紮モデルの髄腔内にBoNT/A を投与し,BoNT/A がvon-Frey 試験による痛み回避行動への作用とパッチクランプ・ホールセル記録法による脊髄後角興奮性シナプス伝達に及ぼす作用を評価した.坐骨神経の部分結紮後に0.15 単位のBoNT/A を髄腔内投与し,von-Frey 試験によって神経障害性疼痛の症状であるアロディニアに対する鎮痛効果を評価した結果,BoNT/A はアロディニアによる痛み回避行動を減少させた.また,BoNT/A 投与7 日後に脊髄スライス標本のパッチクランプ・ホールセル記録法で微小興奮性シナプス後電流(mEPSCs)を観察した結果,BoNT/A は対照と比較してmEPSCs の振幅に変化を生じずに発生頻度を減少させ,単刺激による刺激誘発性興奮性シナプス後電流(eEPSCs)の振幅も抑制した.以上の結果から,BoNT/A の髄腔内投与は脊髄後角興奮性シナプス伝達の増強を,少なくともシナプス前性に抑制することが示唆された., To clarify the analgesic effect in neuropathic pain of botulinum toxin type A( BoNT/A), a study regarding intrathecal administration of BoNT/A in a neuropathic pain model was performed. 0.15 units of intrathecal BoNT/A was administered 2 days after sciatic ligation (Seltzer method). Allodynia was examined using the von Frey test, and the effect of BoNT/A on excitatory synaptic transmission in thespinal dorsal horn was evaluated using patch clamp wholecell recording.The attenuation of allodynia was observed in the BoNT/ A treatment group after ligation with the von-Frey test after the sciatic nerve ligation (p<0.05). Patch clamp whole cell recording of spinal slice was performed 7 days after BoNT/A treatment, and miniature excitatory postsynaptic currents( mEPSCs) were observed. The frequency of mEPSCs was significantly reduced compared with the vehicle group (p<0.05) with no effect on amplitude. The amplitude of evoked excitatory postsynaptic currents (eEPSCs) induced by single stimulation was also inhibited by intrathecal BoNT/A.These results suggested that the increasing of excitatory synaptic transmission in the spinal cord dorsal horn might be suppressed by intrathecal administration of BoNT/A, which is likely to produce presynaptic suppression underlying these analgesic effects., 原著},
 pages = {71--78},
 title = {A 型ボツリヌス毒素は神経障害性疼痛による脊髄興奮性シナプスの増強を抑制する},
 volume = {41},
 year = {2014},
 yomi = {ネモト, コウヘイ}
}