{"created":"2023-06-20T14:25:03.514684+00:00","id":120,"links":{},"metadata":{"_buckets":{"deposit":"784be7b2-3b10-47c6-9490-aeb70da56b55"},"_deposit":{"created_by":3,"id":"120","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"120"},"status":"published"},"_oai":{"id":"oai:dmu.repo.nii.ac.jp:00000120","sets":["81:25"]},"author_link":["346","345","342","339","340","343","341","344"],"item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"78","bibliographicPageStart":"71","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Dokkyo journal of medical sciences"}]}]},"item_2_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"Th1細胞は造血幹細胞移植後の急性移植片対宿主病(graft-versus-hostdisease:GVHD)の発症に深く関与しているといわれている。今回我々はTh1細胞に発現するとされるキモカインリセプターの1つであるCXCR3を測定し,瞬帯血移植で急性GVHDがなぜ軽度なのかを検討した。臍帯血,成人末梢血と移植後患者末梢血中のリンパ球,NK細胞をモノクローナル抗体(抗CD4,抗CD8,抗CD56,抗HLA-DR,抗CD25)で染色し以下の結果を得た。CD^4+T細胞のCXCR3発現,HLA-DRの発現はいずれも末梢血よりも膀帯血の方が少なかった(p<0.05)。NK(CD56^+)細胞ではCXCR3,HLA-DR発現に有意差はなかった。IL-2で刺激したCD4^+T細胞でのCXCR3発現は培養後5日でも謄帯血では末梢血より発現が少なかった(p<0.05)。IL-2で刺激したNK細胞のCXCR3発現には両者に差がなかったが,HLA-DR発現は末梢血と比べて膀帯血で少なかった(p<0.05)。移植1ヶ月後の末梢血CD^+T細胞のCXCR3発現を膀帯血移植例と対照の同種骨髄移植例で比較すると膀帯血移植例で低値であった(p<0.05)。膀帯血のCD^+T細胞.NK細胞でのCXCR3発現の特徴は膀帯血移植で急性GVHDが発症しにくいということに関与していると考えた","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"In order to know why the development of acute graft-ver-sus-host-disease (aGVHD) is significantly milder after stem cell transplantation (SCT) using cord blood, we studied the CXCR3 expression on CD4^+T cells and NK (CD 56^+) cells, based on the fact that CXCR3 is one of the chemokine receptors on Th1 cells. Cord blood (CB) and peripheral blood (PB) from healthy volunTFFrs and patients who received SCT were treated with monoclonal antibodies against CD4, CD25, CD56, CXCR3, and HLA-DR and the antigen expression was analyzed using flow cytometry. The percentage of CXCR3 antigen on GB and PB CD4^+ T cells was 2.6 ± 1.4% and 30.8 ± 3.9%, respectively, demonstrating a significant difference (p < 0.05). However, that on GB and PB NK cells was not significantly different. CXCR3 expression on CB and PB CD4^+T cells stimulated by IL-2 for 72h and 120h was significantly different (p < 0.05), but that on NK cells was not significant. CXCR3 expression on CD4^+ T cells from patients who received allo-CB-SCT and allo-bone marrow transplantation from HLA-mismatched donor was significantly different at one-month post-SCT (p < 0.05). We concluded that the lower CXCR3 expression on GB CD^+T cells and absence of difference in CXCR3 expression on cord blood NK cells facilitated a milder aGVHD.","subitem_description_type":"Other"}]},"item_2_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Original","subitem_description_type":"Other"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00629581"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855023","subitem_source_identifier_type":"ISSN"}]},"item_2_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"獨協医科大学小児科学(血液)"},{"subitem_text_value":"獨協医科大学小児科学(血液)"},{"subitem_text_value":"獨協医科大学小児科学(血液)"},{"subitem_text_value":"獨協医科大学産婦人科学"}]},"item_2_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Pediatrics, Dokkyo University School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Pediatrics, Dokkyo University School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Pediatrics, Dokkyo University School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Obstetrics & Gynecology, Dokkyo University School of Medicine"}]},"item_2_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"サイタイケツ チュウ D4^+ サイボウ, NKサイボウ ノ キモカインリセプター CXCR3 ハツゲン ノ ケントウ"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"坪井, 龍生"},{"creatorName":"ツボイ, タツオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"杉田, 憲一"},{"creatorName":"スギタ, ケンイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"江口, 光興"},{"creatorName":"エグチ, ミツオキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"渡辺, 博"},{"creatorName":"ワタナベ, ヒロシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuboi, Tatsuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugita, Kenichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Eguchi, Mitsuoki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-24"}],"displaytype":"detail","filename":"KJ00000010451.pdf","filesize":[{"value":"681.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000010451.pdf","url":"https://dmu.repo.nii.ac.jp/record/120/files/KJ00000010451.pdf"},"version_id":"67d3ee49-070b-4568-ba2d-cd81c43900ea"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CXCR3"},{"subitem_subject":"CD4^+T細胞"},{"subitem_subject":"NK細胞"},{"subitem_subject":"膀帯血"},{"subitem_subject":"急性移植片対宿主病"},{"subitem_subject":"CXCR3","subitem_subject_language":"en"},{"subitem_subject":"GD4^+T cell","subitem_subject_language":"en"},{"subitem_subject":"Cord Blood","subitem_subject_language":"en"},{"subitem_subject":"Stem Cell Transplantation","subitem_subject_language":"en"},{"subitem_subject":"acute graft-versus-host-disease (aGVHD)","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"臍帯血中CD4^+T細胞,NK細胞のキモカインリセプターCXCR3発現の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"臍帯血中CD4^+T細胞,NK細胞のキモカインリセプターCXCR3発現の検討"},{"subitem_title":"CXCR 3 Expression on Cord Blood CD 4^+ T Cells and NK Cells","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["25"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-05-24"},"publish_date":"2017-05-24","publish_status":"0","recid":"120","relation_version_is_last":true,"title":["臍帯血中CD4^+T細胞,NK細胞のキモカインリセプターCXCR3発現の検討"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T16:22:38.304857+00:00"}