{"created":"2023-06-20T14:25:04.326728+00:00","id":138,"links":{},"metadata":{"_buckets":{"deposit":"de758d82-cad2-4106-a59e-bcd90fa795fc"},"_deposit":{"created_by":3,"id":"138","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"138"},"status":"published"},"_oai":{"id":"oai:dmu.repo.nii.ac.jp:00000138","sets":["81:26"]},"author_link":["436","434","433","438","437","435"],"item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-07-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"151","bibliographicPageStart":"141","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Dokkyo journal of medical sciences"}]}]},"item_2_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Prostaglandin D_2 (PGD_2), a mast cell-derived lipid mediator is detected in lage amounts in airways of asthmatics, but its role of largely unkown. To clarify the role of PGD_2 in Th2-type airway inflammation which characterizes asthma, we studied the effects of aerosolized PGD_2 on the inflammatory response to a low-dose antien challenge in airways of mice. Mice sensitized with ovalbumin (OVA) were challenged with a conventional-dose (1%) or a low dose (0.1%) aerosolized OVA. Mice received low - dose OVA challenge were pretreated with aerosolized PGD_2 (10^<-3>M) (PGD_2 plus low-dose OVA mice) or saline (low-dose OVA alone mice) 24 hrs before the OVA challenge. Some mice were pretreated with PGD_2 but challenged with saline (PGD_2 alone mice). Airway inflammation was evaluated by the numbers of eosinophils, lymphocytes and macrophages in bronchoalveolar lavage fluid. The degree of airway inflammation in the PGD_2 alone mice and the low-dose OVA alone mice were only marginal. However, the PGD_2 plus low-dose OVA mice displayed a similar degree of airway inflammation with mice received conventional-dose OVA challenge. Levels of interleukin (IL)-4 and IL-5 were significantly increased in the PGD_2 plus low-dose OVA mice than the low-dose OVA alone mice. PGD_2 (10^<-9>-10^<-5> M) did not affect the Th2-type cytokine production by OVA specific T cells in response to OVA stimulation in vitro. Immunohistochemical analysis of lung tissue revealed that airway epithelium of the PGD_2 plus low-dose OVA alone mice were strongly stained with monoclonal antibody against macrophage-derived chemokine (MDC), a Th2 cell-specific chemokine. These results suggest that PGD_2 augments Th2 cell -type airway inflammation via epithelial experssion of MDC.","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Original","subitem_description_type":"Other"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00629581"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855023","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medical and Clinical Immunology, Dokkyo University School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medical and Clinical Immunology, Dokkyo University School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medical and Clinical Immunology, Dokkyo University School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medical and Clinical Immunology, Dokkyo University School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medical and Clinical Immunology, Dokkyo University School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medical and Clinical Immunology, Dokkyo University School of Medicine"}]},"item_2_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"Prostaglandin D_2 Augments Low-dose Antigen-induced Th2 Type Airway Inflammation in Mice"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Honda, Kyoko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirata, Hirokuni","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Eda, Fukiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukushima, Fumiya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Bunpei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Arima, Masafumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-24"}],"displaytype":"detail","filename":"KJ00000010469.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000010469.pdf","url":"https://dmu.repo.nii.ac.jp/record/138/files/KJ00000010469.pdf"},"version_id":"164ba1ac-f473-4c0d-bcf8-db39a5ca7e93"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Th2","subitem_subject_language":"en"},{"subitem_subject":"prostaglandin D_2","subitem_subject_language":"en"},{"subitem_subject":"MDC","subitem_subject_language":"en"},{"subitem_subject":"bronchial asthma","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Prostaglandin D_2 Augments Low-dose Antigen-induced Th2 Type Airway Inflammation in Mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Prostaglandin D_2 Augments Low-dose Antigen-induced Th2 Type Airway Inflammation in Mice","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-05-24"},"publish_date":"2017-05-24","publish_status":"0","recid":"138","relation_version_is_last":true,"title":["Prostaglandin D_2 Augments Low-dose Antigen-induced Th2 Type Airway Inflammation in Mice"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T16:23:00.781529+00:00"}