{"created":"2023-06-20T14:26:09.025235+00:00","id":1475,"links":{},"metadata":{"_buckets":{"deposit":"53f0da16-d8e4-49e8-bd95-7750b1f2b9fc"},"_deposit":{"created_by":14,"id":"1475","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"1475"},"status":"published"},"_oai":{"id":"oai:dmu.repo.nii.ac.jp:00001475","sets":["81:86"]},"author_link":["6411","6406","6409","6414","6909","6908","6420","1642","6907"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"38","bibliographicPageStart":"31","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"Dokkyo Journal of Medical Sciences"}]}]},"item_10001_description_34":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"Urate (uric acid) is the major inert end product of purine metabolism in humans. Since it is water soluble, it requires a membranous protein called transporter for its permeation across the plasma membrane. Increased blood urate level is often seen in preeclampsia, but its precise mechanism remains unknown. Syncytiotrophoblasts function as a barrier between maternal blood and fetal one so called “blood-placental barrier”. So far, the expression of several urate transporters was identified in these cells, but it is still unclear about their contribution to urate handling in blood-placental barrier. In this study, we investigated the expression of urate transporters and the properties of ［14C］urate transport in both JAR and JEG-3, human choriocarcinoma cells as a model of human placenta. Conventional PCR analysis revealed that both JAR and JEG-3 cells express strongly breast cancer resistance protein (BCRP/ABCG2) mRNA. Uptake of ［14C］urate by these cells is time-dependent with Na＋- and Cl－-independent and voltage-insensitive manner and is not inhibited by benzbromarone, a representative renal urate transport inhibitor. Then, we focused on BCRP which shows strong mRNA expression and found that these cells have urate efflux property that is sensitive to fumitremorgin C (FMC), a BCRP inhibitor. These results suggest that BCRP is one of the important components for urate handling in human placenta in pathophysiological condition such as preeclampsia.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"獨協医学会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00629581","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855023","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_24":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Pharmacology and Toxicology and Department of Obstetrics and Gynecology, Dokkyo Medical University School of Medicine"},{"subitem_text_value":"Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"},{"subitem_text_value":"Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"},{"subitem_text_value":"Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"},{"subitem_text_value":"Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"},{"subitem_text_value":"Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"},{"subitem_text_value":"Department of Obstetrics and Gynecology, Dokkyo Medical University School of Medicine"},{"subitem_text_value":"Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"},{"subitem_text_value":"Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine:Department of Pharmacology, Chiba University Graduate School of Medicine"}]},"item_10001_text_28":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_text_value":"6"}]},"item_10001_text_33":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_text_value":"Original"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kiuchi, Kaori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Asuka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ouchi, Motoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Otani, Naoyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jutabha, Promsuk","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nohara, Masakatsu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukasawa, Ichio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujita, Tomoe","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Anzai, Naohiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-09"}],"displaytype":"detail","filename":"DJMS-44-1-6.pdf","filesize":[{"value":"206.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DJMS-44-1-Kiuchi-本文","url":"https://dmu.repo.nii.ac.jp/record/1475/files/DJMS-44-1-6.pdf"},"version_id":"998c57ed-325d-4b10-83a4-0ab1c0fafbab"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"urate","subitem_subject_scheme":"Other"},{"subitem_subject":"placenta","subitem_subject_scheme":"Other"},{"subitem_subject":"preeclampsia","subitem_subject_scheme":"Other"},{"subitem_subject":"hyperuricemia","subitem_subject_scheme":"Other"},{"subitem_subject":"transporters","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of Urate Transport System in JAR and JEG-3 Cells, Human Trophoblast-derived Cell Lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of Urate Transport System in JAR and JEG-3 Cells, Human Trophoblast-derived Cell Lines"}]},"item_type_id":"10001","owner":"14","path":["86"],"publish_date":"2018-07-09","publish_status":"0","recid":"1475","relation_version_is_last":true,"title":["Characterization of Urate Transport System in JAR and JEG-3 Cells, Human Trophoblast-derived Cell Lines"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-06-20T15:44:46.751563+00:00"}