{"created":"2023-06-20T14:26:09.972966+00:00","id":1492,"links":{},"metadata":{"_buckets":{"deposit":"81679df0-d144-401c-88dd-81fb9322ecf2"},"_deposit":{"created_by":14,"id":"1492","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"1492"},"status":"published"},"_oai":{"id":"oai:dmu.repo.nii.ac.jp:00001492","sets":["81:87"]},"author_link":["4069","6963","4073"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-07-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"140","bibliographicPageStart":"135","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"Dokkyo Journal of Medical Sciences"}]}]},"item_10001_description_34":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"Purpose：Reactive oxygen species have been suggested as a major contributing factor to cryodamage of the spermatozoa. Accordingly, antioxidant supplementation has been used to yield significantly improved quality of frozen sperm post-thaw. We sought to investigate outcomes with a combination of antioxidants\ncatalase and N-acetylcysteine respectively. \n\nMethods：The cryomedium supplemented with 200U/ml catalase, 5 mM N-acetylcysteine and their combination was used. Semen samples were collected from normozoospermic men （n＝20）and aliquots frozen in each dose of antioxidant tested. Post-thaw semen analysis by computer aided sperm analysis was performed. In addition, sperm viability （Eosin-Y staining） and sperm DNA fragmentation（TUNEL assay）were quantitated. \n\nResults：Combined catalase and N-acetylcysteine supplementation during cryopreservation resulted in significantly improved post-thaw recovery of total motility（from 39.4±15.8％ to 49.2±16.1％） and percentage of sperm vitality （from 46.2±13.3％ to 53.2±11.8％）（mean±SD, p＜0.05）. Catalase improved post-thaw DNA integrity analysis for doublestranded breaks using TUNEL assay. \n\nConclusions：These results indicated that the combination of intracellular and extracellular antioxidants resulted in the pronounced effect in improving post-thaw quality of human spermatozoa.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"獨協医学会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00629581","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855023","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_24":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Urology, University of Illinois at Chicago:Department of Urology, Dokkyo Medical University Koshigaya Hospital"},{"subitem_text_value":"Department of Urology, Dokkyo Medical University Koshigaya Hospital"},{"subitem_text_value":"Department of Urology, University of Illinois at Chicago"}]},"item_10001_text_28":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_text_value":"2"}]},"item_10001_text_33":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_text_value":"Original"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kobori, Yoshitomo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okada, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Niederberger, Craig","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-10"}],"displaytype":"detail","filename":"DJMS-44-2-2.pdf","filesize":[{"value":"75.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DJMS-44-2-Kobori-本文","url":"https://dmu.repo.nii.ac.jp/record/1492/files/DJMS-44-2-2.pdf"},"version_id":"dcf79d5a-1fc4-4a5d-b88f-6e7e230de34b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Antioxidant","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA damage","subitem_subject_scheme":"Other"},{"subitem_subject":"Sperm cryopreservation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Supplementation of Cryomedium with Catalase and N-acetylcysteine Improves Human Sperm Post-thaw Motility and DNA Integrity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Supplementation of Cryomedium with Catalase and N-acetylcysteine Improves Human Sperm Post-thaw Motility and DNA Integrity"}]},"item_type_id":"10001","owner":"14","path":["87"],"publish_date":"2018-07-10","publish_status":"0","recid":"1492","relation_version_is_last":true,"title":["Supplementation of Cryomedium with Catalase and N-acetylcysteine Improves Human Sperm Post-thaw Motility and DNA Integrity"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-06-20T15:45:17.049107+00:00"}