| Item type |
学術雑誌論文 / Journal Article(1) |
| タイトル |
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|
タイトル |
Comparison and Molecular Localization Analysis of Porcine and Human Skin Lipids by High-resolution Imaging Mass Spectrometry |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cholesterol sulfate |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
imaging mass spectrometry |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
lipid |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
mass spectrometry |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
matrix assisted laser desorption ionization |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Tokoro, Shown
Ogawa, Tadayuki
Hayashi, Shujiro
Igawa, Ken
|
| 書誌情報 |
en : Dokkyo Medical Journal
巻 4,
号 2,
p. 107-116,
発行日 2025-04-25
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| 記事種別 |
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|
値 |
Original |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Lipidomics research has accelerated in recent years because of advances in mass spectrometry technology. Imaging mass spectrometry (IMS) is a powerful technique to obtain comprehensive molecular information for identification of lipids and their localization in tissues to explore the underlying pathogenesis of skin diseases, including atopic dermatitis, asteatosis, and ichthyosis. The aim of this study was to compare and perform molecular localization analysis of porcine and human skin lipids by high-resolution matrix assisted laser desorption/ ionization (MALDI)-IMS using 9-Aminoacridine (9AA) matrix. 9AA was vapor-deposited onto the thinly sliced skin samples as a matrix to promote ionization of the analyte molecule following laser irradiation. We successfully visualized various skin lipids of both species localized in three different skin layers (stratum corneum, viable epidermis, and dermis) by this method. Molecules with peaks at m/z 465.305, 474.361, and 659.394 were mainly localized in the stratum corneum, those with peaks at m/z 184.076, 273.042, 378.159, 570.228, and 579.228 were localized in the viable epidermis, and those with peaks at m/z 607.361, 615.201, 659.192, 687.545, and 703.571 were localized in the dermis in both species. However, peaks at m/z 465.305 and 659.394 were assigned to cholesterol sulfate and were localized exclusively in the stratum corneum in porcine skin, but abundant in the granular layer in human skin. In conclusion, we performed MALDI-IMS using 9AA matrix and found most of the lipid molecules in porcine and human skin showed similar distributions. However, there were some differences including the localization of cholesterol sulfate. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Dokkyo Medical Society |
| ISSN |
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|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2436-522X |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12941861 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.51040/dkmj.2023-068 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
DKMJ-4-2-2.pdf (2.2 MB)
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