@article{oai:dmu.repo.nii.ac.jp:00002102,
 author = {Imanishi, Masaya and Asato, Hirotaka and Umekawa, Kohei and Sasaki, Shoichi and Terashima, Tetsuji},
 issue = {3},
 journal = {Dokkyo Journal of Medical Sciences, Dokkyo Journal of Medical Sciences},
 month = {Oct},
 note = {Purpose：Several studies investigated the efficacy of transplanting adipose-derived mesenchymal stem cells（ADMSCs）in the treatment of neuropathic pain in animals. However, these studies evaluated the effects of ADMSCs transplantation by assessing the mechanical allodynia but not spontaneous pain. Here, we verify whether ADMSCs transplantation improves spontaneous pain in a rat model of neuropathic pain. 
Methods：ADMSCs were isolated from rat adipose tissue and cultured. Chronic constriction injury（CCI） model rats were created by surgical maneuver. A total of 20 F344 rats underwent surgery and were divided into 2 groups, the ADMSCs group and the control group. One week after CCI surgery, ADMSCs were transplanted into the epineurium of the damaged nerve. The effects of ADMSCs transplantation were evaluated by the number of spontaneous pain-related behaviors and the degree of mechanical allodynia. The degree of mechanical allodynia was assessed with the von-Frey filament test. 
Results：No rats died during the experiments and all CCI model rats were established successfully. ADMSCs transplantation improved mechanical allodynia on and after 7-day post-transplantation and spontaneous pain on and after 21-day post-transplantation with the statistically significant differences. These improvement effects were observed until 6-week post-transplantation in mechanical allodynia and 5-week post-transplantation in spontaneous pain. 
Conclusion：ADMSCs transplantation improved not only mechanical allodynia but also spontaneous pain. ADMSCs transplantation may be an effective treatment for neuropathic pain in clinical practice.},
 pages = {119--125},
 title = {Adipose-derived Mesenchymal Stem Cells Improve Both Spontaneous Pain and Allodynia in a Rat Neuropathic Pain Model},
 volume = {45},
 year = {2018}
}