{"created":"2023-06-20T14:26:32.649653+00:00","id":2114,"links":{},"metadata":{"_buckets":{"deposit":"32aeb6bc-8ba9-4c25-bf27-bc73caeb16f6"},"_deposit":{"created_by":3,"id":"2114","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2114"},"status":"published"},"_oai":{"id":"oai:dmu.repo.nii.ac.jp:00002114","sets":["81:156"]},"author_link":["9820","9818","9819"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-07-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"51","bibliographicPageStart":"43","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"Dokkyo Journal Of Medical Sciences"}]}]},"item_10001_description_34":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"　RUNX1 and RUNX3 are master transcription factors in sensory neuron lineage specifications. Protein levels of such developmental regulators are tightly controlled during carcinogenesis, in order to block differentiation and drive proliferation. Here we report that neuroblastoma specific microRNAs inhibit protein syntheses of RUNX1 and RUNX3 through 3’UTR sequences. Computational prediction identified two putative binding sequences for N-Myc-activated microRNAs both in RUNX1 and RUNX3 3’UTRs. Streptavidin RNA aptamer-tagged 3’UTR sequences pulled down miR-17, miR-18a, miR-19a, miR-20a or miR-130a from neuroblastoma cell lysate. 3’UTR target protection from N-Myc-activated microRNAs increased protein synthesis of RUNX1 or RUNX3 and induced differentiation in neuroblastoma cell lines. Together, protein levels of RUNX1 and RUNX3 are post-transcriptionally regulated by N-Myc-activated microRNAs, highlighting the mutual negative feedback between N-Myc oncogene and RUNX3 tumor suppressor in neuroblastoma.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"獨協医学会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00629581 ","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855023 ","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_24":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Research Support Center, Dokkyo Medical University School of Medicine, Mibu, Japan"},{"subitem_text_value":"Research Support Center, Dokkyo Medical University School of Medicine, Mibu, Japan"},{"subitem_text_value":"Research Support Center, Department of Cardiovascular Medicine, Dokkyo Medical University School of Medicine, Mibu, Japan"}]},"item_10001_text_33":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_text_value":"Original"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Inoue, Ken-ichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akimoto, Kazumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inoue, Teruo","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-02-06"}],"displaytype":"detail","filename":"DJMS-45-2-2.pdf","filesize":[{"value":"364.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DJMS-45-2-Inoue-本文","url":"https://dmu.repo.nii.ac.jp/record/2114/files/DJMS-45-2-2.pdf"},"version_id":"ee4b4057-ef7e-4d2d-ba6c-5f8848b17184"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Neuroblastoma","subitem_subject_scheme":"Other"},{"subitem_subject":"RUNX transcription factor","subitem_subject_scheme":"Other"},{"subitem_subject":"N-Myc","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptavidin RNA aptamer","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"N-Myc-activated microRNAs Inhibit Protein Synthesis of RUNX1 and RUNX3 in Neuroblastoma Cell Lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"N-Myc-activated microRNAs Inhibit Protein Synthesis of RUNX1 and RUNX3 in Neuroblastoma Cell Lines"}]},"item_type_id":"10001","owner":"3","path":["156"],"publish_date":"2019-02-06","publish_status":"0","recid":"2114","relation_version_is_last":true,"title":["N-Myc-activated microRNAs Inhibit Protein Synthesis of RUNX1 and RUNX3 in Neuroblastoma Cell Lines"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T15:36:03.785868+00:00"}