{"created":"2023-06-20T14:25:08.980788+00:00","id":213,"links":{},"metadata":{"_buckets":{"deposit":"8888aad9-779d-4940-b1c3-dac84024c454"},"_deposit":{"created_by":3,"id":"213","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"213"},"status":"published"},"_oai":{"id":"oai:dmu.repo.nii.ac.jp:00000213","sets":["81:29"]},"author_link":["740"],"item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"16","bibliographicPageStart":"7","bibliographicVolumeNumber":"31","bibliographic_titles":[{"bibliographic_title":"Dokkyo journal of medical sciences"}]}]},"item_2_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Cytocidal effects of anti-cancer drugs such as cisplatin, 5-fluorouracil (5-FU), and actinomycin D on various cell lines were investigated using Alamar Blue. Actinomycin D, cisplatin and 5-FU showed significant cytocidal effects at 1μg/ml, 10μg/ml and 50μg/ml respectively, on cultured cells after 24 h incubation at 37℃. The cytocidal effects increased at a low cell density but reduced at a high cell density in various cell lines. The expression level of one of the ribosomal proteins, nbl (S3a), was measured by northern blot analysis and real time-polymerase chain reaction. The relationship between the nbl mRNA level and drug effects was then investigated for 8 rat and 10 human cell lines. An inverse correlation between chemosensitivity and nbl mRNA level was observed in both rat and human cell lines.","subitem_description_type":"Other"}]},"item_2_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Original","subitem_description_type":"Other"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00629581"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855023","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"First Department of Surgery, Dokkyo University School of Medicine"}]},"item_2_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"Studies on Relationship between nbl (S3a) mRNA Levels, Cell Density, and Chemosensitivity in Various Rat and Human Cell Lines"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nemoto, Kazuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-24"}],"displaytype":"detail","filename":"KJ00004091306.pdf","filesize":[{"value":"949.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004091306.pdf","url":"https://dmu.repo.nii.ac.jp/record/213/files/KJ00004091306.pdf"},"version_id":"3d55b1d4-9ed0-453b-be7d-263d2478de94"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cultured cells","subitem_subject_language":"en"},{"subitem_subject":"Chemosensitivity","subitem_subject_language":"en"},{"subitem_subject":"nbl (S3a)","subitem_subject_language":"en"},{"subitem_subject":"Cell density","subitem_subject_language":"en"},{"subitem_subject":"Alamar Blue","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Studies on Relationship between nbl (S3a) mRNA Levels, Cell Density, and Chemosensitivity in Various Rat and Human Cell Lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Studies on Relationship between nbl (S3a) mRNA Levels, Cell Density, and Chemosensitivity in Various Rat and Human Cell Lines","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-05-24"},"publish_date":"2017-05-24","publish_status":"0","recid":"213","relation_version_is_last":true,"title":["Studies on Relationship between nbl (S3a) mRNA Levels, Cell Density, and Chemosensitivity in Various Rat and Human Cell Lines"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T16:24:52.899901+00:00"}