{"created":"2023-06-20T14:26:45.614311+00:00","id":2419,"links":{},"metadata":{"_buckets":{"deposit":"f4cb2de3-03de-47c5-83b5-cedf8b0306bf"},"_deposit":{"created_by":14,"id":"2419","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"2419"},"status":"published"},"_oai":{"id":"oai:dmu.repo.nii.ac.jp:00002419","sets":["81:189"]},"author_link":["10817","10816","10815"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-10-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"123","bibliographicPageStart":"115","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"Dokkyo Journal of Medical Sciences"}]}]},"item_10001_description_35":{"attribute_name":"要旨(英)","attribute_value_mlt":[{"subitem_description":"  Neuroblastoma （NB） is the most common pediatric abdominal solid tumor with less than 50％ of longterm survival rate in high-risk cases. Ring finger protein 1/2, referred to as RING1A/B respectively, are E3 ubiquitin ligases that compose a catalytic subunit of Polycomb Repressive Complex-1 （PRC1）. PRC1 epigenetically down-regulates target gene transcription, especially tumor suppressor genes in tumorigenesis and cancer progression. However, the function of RING1 proteins is still unclear in NB. This study aims to explore the importance of RING1A （gene name, RING1） in human NB cells to evaluate its druggability.\n  According to the Kaplan-Meier analysis based on the public NB patients’ transcript data, lower expression of RING1 showed poor prognosis. As such, we hypothesized the presence of the anti-tumorigenic function of RING1A. First, mouse Ring1A （mRing1A；gene name, Ring1） was transiently expressed in a NB cell line, NGP. The subsequent flat colony formation assay resulted in a decreased number of colonies, and intriguingly, endogenous RING1B expression was dampened in the transfectants. On top of that, we established NGP cells with inducible short hairpin （sh） RNA against RING1. Unexpectedly, short hairpin （sh） RING1 induction repressed cell proliferation. However, consistent with the transient expression of mRing1A, endogenous RING1B expression was elevated up on the shRING1 induction.\n  Here, we showed that both gain and loss of RING1A expression suppressed NGP cell growth, and RING1A negatively controlled RING1B expression. Although the druggability of RING1A is still unclear, the current study suggests that RING1A does not functionally compensate RING1B, and the optimal expression of RING1A is essential for NB cell proliferation.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"獨協医学会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00629581","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855023","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_25":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Pediatric Surgery, Dokkyo Medical University Saitama Medical Center"},{"subitem_text_language":"en","subitem_text_value":"Research Institute for Clinical Oncology, Saitama Cancer Center"},{"subitem_text_language":"en","subitem_text_value":"Research Institute for Clinical Oncology, Saitama Cancer Center"}]},"item_10001_text_33":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_text_value":"Original"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hasegawa, Mariko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satoh, Shunpei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kamijo, Takehiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-01-06"}],"displaytype":"detail","filename":"DJMS-47-3-2.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DJMS-47-3-Hasegawa-本文","url":"https://dmu.repo.nii.ac.jp/record/2419/files/DJMS-47-3-2.pdf"},"version_id":"19b46e4c-e1a3-45d7-adbe-fd70d481e50d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"RING1A","subitem_subject_scheme":"Other"},{"subitem_subject":"RING1B","subitem_subject_scheme":"Other"},{"subitem_subject":"neuroblastoma","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"RING1A Regulates RING1B Expression and the Collapse of its Expression Impairs Neuroblastoma Cell Proliferation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"RING1A Regulates RING1B Expression and the Collapse of its Expression Impairs Neuroblastoma Cell Proliferation"}]},"item_type_id":"10001","owner":"14","path":["189"],"publish_date":"2021-01-06","publish_status":"0","recid":"2419","relation_version_is_last":true,"title":["RING1A Regulates RING1B Expression and the Collapse of its Expression Impairs Neuroblastoma Cell Proliferation"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-06-20T15:25:42.238266+00:00"}