@article{oai:dmu.repo.nii.ac.jp:00002504,
 author = {Kan, Takeshi and Asato, Hirotaka and Fukuda, Norio and Takada, Goro and Sasaki, Shoichi},
 issue = {1},
 journal = {Dokkyo Journal of Medical Sciences},
 month = {Mar},
 note = {Hepatocyte growth factor（HGF）has a strong angiogenic effect on various organs. Rapid administration of intravenous heparin increases human plasma HGF concentration. Angiogenesis has a positive effect on flap survival, and the angiogenic effect of administered heparin is expected to expand the flap survival area. Previous reports stated that heparin administration could expand the flap survival area, but they did not focus on the angiogenesis effect of heparin but instead its anti-coagulation and anti-inflammatory effects. Therefore, we studied the effects of heparin administration on flap survival in an animal model and expected its angiogenic effect to expand the flap survival area. Twenty male Wistar rats（8-9 weeks old）were randomly divided into two groups of ten：heparin and control groups. Distally based McFarlane flaps were raised in all rats. In the heparin group, 300 U/kg of heparin was administered daily, whereas, in the control group, saline was administered daily. We compared the flap survival area and the number of new blood vessels on postoperative day 7. The mean flap survival areas were significantly higher in the heparin group（57.9±5.3％）than in the control group（47.3±5.9％）（P＜0.01）. The number of CD31-positive cells in ten high power field images was significantly higher in the heparin group（60.3±7.3 cells）than in the control（43.7±4.8 cells）（P＜0.01）. In our murine model, heparin administration positively affected flap survival and angiogenesis. We consider that the increased serum HGF concentration via heparin administration is responsible for this result.},
 pages = {43--49},
 title = {Heparin Administration Expands Flap Survival Area Possibly by Increasing the Concentration of Hepatocyte Growth Factor},
 volume = {48},
 year = {2021}
}