@article{oai:dmu.repo.nii.ac.jp:00000323,
 author = {吉井, 直子 and Yoshii, Naoko},
 issue = {1},
 journal = {Dokkyo journal of medical sciences},
 month = {Mar},
 note = {Serine orotease inhibitorの1つであるulinastatinの腫瘍における血管新生および血行性転移に対する効果について検討した.方法・結果:1) 肺癌細胞A549又はEBC-1を注入したchamberをマウス背部皮下に植え込んだ.Ulinastatin投与群(U群)と非投与対照群(C群)に分けた.7日後のA549及びEBC-1による皮下新生血管平均総長は,U群はC群に比し有意に減少した.2) EBC-1をマウス背部皮下に移植し,PBS(P群)又はulinastatin(U群)を7日間連続投与した.15日目の腫瘍のCD31免疫染色で,腫瘍内血管数は,U群はP群に比し有意に減少した.TUNEL assayによる腫瘍内アポトーシス細胞数は,U群はP群に比し有意に増加した.3) A549をマウス尾静脈より注入し,注入日よりPBS(P群)またはulinastatin(U群)を14日間連続投与した.29日目のCEA免疫染色による肺転移面積は,U群はP群に比し有意に減少した.結論:Ulinastatinは腫瘍血管新生を抑制し,腫瘍細胞死を誘導した.また,転移抑制効果も認められた., We investigated effects of ulinastatin, one of serine protease inhibitors, on tumor cell induced angiogenesis and lung metastasis. Methods : 1) Lung cancer cell line, A549 or EEC-1, was injected into a chamber, which was implanted in the dorsum of mice. The mice were divided into 2 groups in each tumor cell line, based on 7-day administration of ulinastatin, with (U group) or without it (C group). The chamber with surrounding subcutaneous tissues was extirpated and newly induced blood vessels were observed with a microscope. The image was taken into a computer and the total length of blood vessels (TLBV) was measured. 2) EEC-1 was implanted in the dorsum of mice subcutaneously, in which PBS (P group) or ulinastatin (U group) was injected from day 8 to day 14. The grown tumors were extirpated on day 15 and the specimens were stained with CD31 or TUNEL assay. 3) A549 was injected into a tail vein of the mice, which were divided into 2 groups based on 14-day administration of PBS (P group) or ulinastatin (U group). On the day 29, the lung was extirpated and area of lung metastasis stained with CEA was measured. Results : 1) Each tumor cell line induced TLBV was significantly shorter in U group than C group. 2) Number of CD31 stained blood vessels in tumor was significantly smaller in U group than P group. Number of apoptotic tumor cells stained with TUNEL assay was significantly larger in U group than P group. 3) The area of lung metastasis was significantly smaller in U group than P group. Conclusion : Ulinastatin inhibited tuomr cell induced angiogenesis. It is suggested that this inhibitory effect on angiogenesis led to tumor cell deaths and decrease in lung metastasis., 原著, Original},
 pages = {47--56},
 title = {Ulinastatinの腫瘍における血管新生に対する抑制効果},
 volume = {32},
 year = {2005},
 yomi = {ヨシイ, ナオコ}
}