{"created":"2023-06-20T14:25:19.539218+00:00","id":440,"links":{},"metadata":{"_buckets":{"deposit":"32e5873e-e0d3-4b88-a8ef-aef9ba1d4b67"},"_deposit":{"created_by":3,"id":"440","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"440"},"status":"published"},"_oai":{"id":"oai:dmu.repo.nii.ac.jp:00000440","sets":["81:38"]},"author_link":["1769","1773","1777","1770","1782","1775","1776","1780","1781","1772","1771","1774","1779","1778"],"item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-07-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"132","bibliographicPageStart":"125","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"Dokkyo journal of medical sciences"}]}]},"item_2_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"気管支喘息の基本病態は好酸球,T細胞(特にTh2細胞),肥満細胞,などの炎症細胞を中心とする慢性の気道炎症と気道リモデリングによって矛盾なく説明できる.気道リモデリングの分子レベルでの形成機序は未だ不明な部分が多く,最近線維化サイトカインTGF-βの役割が注目されている.特にシグナル伝達分子Smadの制御機構の不均衡により起こって来ることも解明されつつある.今回我々はTh2サイトカインIL-5,GM-CSFおよび調節性サイトカインIL-10が気道上皮細胞における抑制型Smad7発現に与える影響についてReal time RT-PCR法を用いて検討した.IL-10刺激は,コントロールに比較して,Smad7の発現が増強し,一方でIL-5,GM-CSFはその発現は誘導されなかった.また,その発現はIL-10との混合培養にて増強した.更に,IL-10はSmad7発現を減弱させることが知られている遺伝子TIEG発現に関しても抑制することを見出し,IL-10が制御性サイトカインとして気道炎症の抑制に関与していることが示唆された","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Transforming growth factor-β (TGF-β) is an important pro-fibrogenic growth factor implicated in airway remodeling and pulmonary fibrosis. TGF-β signals from membrane to nucleus by using Smad proteins. Recently, Smad7 has been identified as an intracellular antagonist for TGF-β signaling and it inhibits TGF-β-induced transcriptional responses. And InterleukinlO (IL-10) is general inhibitor of proliferative and cytokine responses in T cells and is produced by mononuclear phagocytes, natural killer cells and by both Th1 and Th2 type lymphocytes. Therefore, we examined the relationship between expression of smad7 in cultured epithelial cells (Beads 2B) stimulated with IL-10. We thought to determine the relationships between Smad7 expression in human bronchial cell line, BEAS-2B cells stimulated with Th2 type cytokine and regulatory cytokine IL-10. Expression levels of Smad7 was expressed in cultured epithelial cells. Interestingly, Th2 cytokines IL-5, GM-CSF exhibited less Smad7 expression in BEAS-2B cells than IL-10 stimulation. In addition, combination with IL-10 and IL-5 stimulation inhibited decreased Smad7 expression. TGF-β induced TIEG expression in BRAS-2B cells were also decreased in a IL-10 stimulation. These findings suggest that Smad7 in a key molecule that defines the susceptibility of bronchial epithelial cells to TGF-β action and regulation of Smad7 expression in bronchial epithelial cells may be related to the development of airway remodeling.","subitem_description_type":"Other"}]},"item_2_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Original","subitem_description_type":"Other"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00629581"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855023","subitem_source_identifier_type":"ISSN"}]},"item_2_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"獨協医科大学内科学:上武呼吸器科内科病院"},{"subitem_text_value":"獨協医科大学内科学"},{"subitem_text_value":"獨協医科大学内科学"},{"subitem_text_value":"獨協医科大学内科学:上武呼吸器科内科病院"},{"subitem_text_value":"獨協医科大学内科学"},{"subitem_text_value":"上武呼吸器科内科病院"},{"subitem_text_value":"獨協医科大学医学総合研究所"}]},"item_2_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medicine and Clinical Immunology, Dokkyo Medical University : Jobu Hospital for Respiratory Disease"},{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medicine and Clinical Immunology, Dokkyo Medical University"},{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medicine and Clinical Immunology, Dokkyo Medical University"},{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medicine and Clinical Immunology, Dokkyo Medical University : Jobu Hospital for Respiratory Disease"},{"subitem_text_language":"en","subitem_text_value":"Department of Pulmonary Medicine and Clinical Immunology, Dokkyo Medical University"},{"subitem_text_language":"en","subitem_text_value":"Jobu Hospital for Respiratory Disease"},{"subitem_text_language":"en","subitem_text_value":"Institute for Medical Science, Dokkyo Medical University"}]},"item_2_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"バイヨウ ヒト キカンシ ジョウヒ サイボウ ニオケル カクシュ サイトカイン シゲキ ガ TGF-βシグナルニ オヨボス エイキョウ "}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"笛木, 直人"},{"creatorName":"フエキ, ナオト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"太田, 真弓"},{"creatorName":"オオタ, マユミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岡田, 壮令"},{"creatorName":"オカダ, タケノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"笛木, 真"},{"creatorName":"フエキ, マコト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"相良, 博典"},{"creatorName":"サガラ, ヒロノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"牧野, 荘平"},{"creatorName":"マキノ, ソウヘイ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"秋元, 一三"},{"creatorName":"アキモト, カズミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fueki, Naoto","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ota, Mayumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okada, Takenori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fueki, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sagara, Hironori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Makino, Sohei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akimoto, Kazumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-24"}],"displaytype":"detail","filename":"KJ00004701720.pdf","filesize":[{"value":"648.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004701720.pdf","url":"https://dmu.repo.nii.ac.jp/record/440/files/KJ00004701720.pdf"},"version_id":"d59f212d-978b-4804-873d-3cb9935d743b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"TGF-βシグナル"},{"subitem_subject":"Smad7"},{"subitem_subject":"気管支喘息"},{"subitem_subject":"IL-10"},{"subitem_subject":"TGF-β signaling","subitem_subject_language":"en"},{"subitem_subject":"Bronchial asthma","subitem_subject_language":"en"},{"subitem_subject":"IL-10","subitem_subject_language":"en"},{"subitem_subject":"Smad7","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"培養ヒト気管支上皮細胞における各種サイトカイン刺激がTGF-βシグナルに及ぼす影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"培養ヒト気管支上皮細胞における各種サイトカイン刺激がTGF-βシグナルに及ぼす影響"},{"subitem_title":"Various Cytokines Regulate TGF-β Signaling in Cultured Airway Epithelial Cells","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["38"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-05-24"},"publish_date":"2017-05-24","publish_status":"0","recid":"440","relation_version_is_last":true,"title":["培養ヒト気管支上皮細胞における各種サイトカイン刺激がTGF-βシグナルに及ぼす影響"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T16:20:39.840121+00:00"}