@article{oai:dmu.repo.nii.ac.jp:00005007,
 author = {Sato-Yazawa, Hanako and Kashiwagi, Korehito and Ishii, Jun and Masawa, Meitetsu and Onozaki, Masato and Miyata-Hiramatsu, Chie and Miyazawa, Tadasuke and Sakaguchi, Mai and Yazawa, Takuya},
 issue = {2},
 journal = {Dokkyo Journal of Medical Sciences},
 month = {Jul},
 note = {In recent decades, genetic engineering and its application to medical sciences has been rapidly progressing, and gene transfection and genome-editing techniques are generally used even in medical investigations. Although establishing single-cell clones are essential for confirming the cellular phenotypic changes in association with genetic modifications, it is technically hard to dispense single-cell efficiently and correctly. Here, we demonstrate single-cell cloning of genetically modified lung cancer cells using a single particle isolation system, On-chip SPiS, by which bi-allelic gene-knockout cells and highly gene-expressing transfectants can be easily and certainly established. In our experiment, we obtained one bi-allelic knockout clone from only 12 clones（8.3％）and eight highly transgene-expressing clones from 20 clones（40％）. Furthermore, we could define that considerable rate of clones without transgene were mixed among selection marker-resistant cell populations. These results suggest that the single-cell dispensing system is a quite useful tool for single-cell cloning in genome-editing and gene-transfection experiments.},
 pages = {75--80},
 title = {Utility of a Single Particle Isolation System in Genome-editing and Gene-transfection Experiments},
 volume = {48},
 year = {2021}
}