{"created":"2023-06-20T14:29:00.651233+00:00","id":5007,"links":{},"metadata":{"_buckets":{"deposit":"82dc21ac-dde1-40ff-b33f-ef9c5515fbf2"},"_deposit":{"created_by":14,"id":"5007","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5007"},"status":"published"},"_oai":{"id":"oai:dmu.repo.nii.ac.jp:00005007","sets":["81:220"]},"author_link":["10412","46321","46316","46318","6439","46320","46317","46322","46319"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-07-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"80","bibliographicPageStart":"75","bibliographicVolumeNumber":"48","bibliographic_titles":[{"bibliographic_title":"Dokkyo Journal of Medical Sciences"}]}]},"item_10001_description_34":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"  In recent decades, genetic engineering and its application to medical sciences has been rapidly progressing, and gene transfection and genome-editing techniques are generally used even in medical investigations. Although establishing single-cell clones are essential for confirming the cellular phenotypic changes in association with genetic modifications, it is technically hard to dispense single-cell efficiently and correctly. Here, we demonstrate single-cell cloning of genetically modified lung cancer cells using a single particle isolation system, On-chip SPiS, by which bi-allelic gene-knockout cells and highly gene-expressing transfectants can be easily and certainly established. In our experiment, we obtained one bi-allelic knockout clone from only 12 clones（8.3％）and eight highly transgene-expressing clones from 20 clones（40％）. Furthermore, we could define that considerable rate of clones without transgene were mixed among selection marker-resistant cell populations. These results suggest that the single-cell dispensing system is a quite useful tool for single-cell cloning in genome-editing and gene-transfection experiments.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"獨協医学会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00629581","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03855023","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_24":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine"},{"subitem_text_value":"Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine"},{"subitem_text_value":"Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine"},{"subitem_text_value":"Department of Respiratory Medicine, Dokkyo Medical University School of Medicine and Graduate School of Medicine"},{"subitem_text_value":"Department of Diagnostic Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine"},{"subitem_text_value":"Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine"},{"subitem_text_value":"Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine"},{"subitem_text_value":"Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine"},{"subitem_text_value":"Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine"}]},"item_10001_text_33":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_text_value":"Original"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato-Yazawa, Hanako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"46316","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kashiwagi, Korehito","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"46317","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishii, Jun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"46318","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masawa, Meitetsu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10412","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Onozaki, Masato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6439","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyata-Hiramatsu, Chie","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"46319","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyazawa, Tadasuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"46320","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakaguchi, Mai","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"46321","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yazawa, Takuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"46322","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-09-14"}],"displaytype":"detail","filename":"DJMS-48-2-4.pdf","filesize":[{"value":"238.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DJMS-48-2-Sato-Yazawa-本文","url":"https://dmu.repo.nii.ac.jp/record/5007/files/DJMS-48-2-4.pdf"},"version_id":"3127b8bd-61d1-4d66-bb73-7e1756f703da"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Single particle isolation system","subitem_subject_scheme":"Other"},{"subitem_subject":"Genome-editing","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene-transfection","subitem_subject_scheme":"Other"},{"subitem_subject":"Single-cell cloning","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Utility of a Single Particle Isolation System in Genome-editing and Gene-transfection Experiments","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Utility of a Single Particle Isolation System in Genome-editing and Gene-transfection Experiments"}]},"item_type_id":"10001","owner":"14","path":["220"],"publish_date":"2021-09-14","publish_status":"0","recid":"5007","relation_version_is_last":true,"title":["Utility of a Single Particle Isolation System in Genome-editing and Gene-transfection Experiments"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-06-20T14:52:29.580890+00:00"}