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PCR for Undiagnosed Sporotrichosis using DNA from Paraffin-embedded Skin Specimens：A Case Study Report from a Single Center
https://dmu.repo.nii.ac.jp/records/5084
https://dmu.repo.nii.ac.jp/records/508485475d6c-129e-4525-9d5a-6622bace75c8
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
DJMS-48-3-Kaminaga-本文 (239.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| タイトル | ||||||||||||||
| タイトル | PCR for Undiagnosed Sporotrichosis using DNA from Paraffin-embedded Skin Specimens：A Case Study Report from a Single Center | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
Kaminaga, Tomoko
× Kaminaga, Tomoko
× Hayashi, Shujiro
× Koike, Masami
× Igawa, Ken
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| 著者所属 | ||||||||||||||
| Department of Dermatology, Dokkyo Medical University | ||||||||||||||
| 著者所属 | ||||||||||||||
| Department of Dermatology, Dokkyo Medical University | ||||||||||||||
| 著者所属 | ||||||||||||||
| Department of Dermatology, Dokkyo Medical University | ||||||||||||||
| 著者所属 | ||||||||||||||
| Department of Dermatology, Dokkyo Medical University | ||||||||||||||
| 書誌情報 |
Dokkyo Journal of Medical Sciences 巻 48, 号 3, p. 143-149, 発行日 2021-10-25 |
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| 要旨 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | Background：The gold standard for diagnosing cutaneous sporotrichosis involves microbiological culture. However, occasionally, culture returns false negatives. In other patients, when mycosis is not suspected, culture specimens are not collected. For example, there are cases in which this disease is not suspected initially because it is difficult to distinguish these lesions from similar manifestations that result from other infectious agents and cutaneous neoplastic lesions. We present a representative case, which imitated squamous epithelial carcinoma clinically and histopathologically. Recently, our group performed nested PCR of DNA extracted from formalin-fixed and paraffin-embedded tissues using Sporothrix-specific primers. Objectives：To assess the clinical utility of this method among cases in which deep cutaneous sporotrichosis was not suspected before skin biopsy, so specimens were not collected, and in cases with clinically suspected but culture-negative sporotrichosis. Materials & Methods：Biopsy specimens were collected from three groups：suspected cutaneous sporotrichosis cases that were histology-positive, but not previously sent for culture；clinically suspected cases, histology-positive and culture-negative；and clinically suspected cases, histology-negative and culturenegative. PCR was performed on these formalin-fixed and paraffin-embedded（FFPE）biopsy samples. Results：Among the cases for which initial cultures were not submitted, 81.3％ were positive for sporotrichosis. In the groups with clinically suspected but culture-negative sporotrichosis, there was also some sporotrichosis detected. Discussions：For patients with negative-culture results, or in situations in which specimens have not been submitted for culture, this method might be useful for diagnosis. Because this was a single-center study, further research on this method at other facilities appears warranted. |
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| 記事種別 | ||||||||||||||
| Original | ||||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | 獨協医学会 | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 03855023 | |||||||||||||
| 書誌レコードID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA00629581 | |||||||||||||
