@article{oai:dmu.repo.nii.ac.jp:00005085,
 author = {Tsuji, Ei-ichi and Ogawa, Toshihisa and Hayashibara, Noriaki and Kiyomatsu, Yuko and Yamaguchi, Nanaka and Nishio, Miki and Hoshi, Yukari and Fujiwara, Hanako and Yoshikata, Marie and Niwa, Takayoshi and Shirakawa, Kazuo},
 issue = {3},
 journal = {Dokkyo Journal of Medical Sciences},
 month = {Oct},
 note = {Background：Ischemic preconditioning（IP）reduces oxidative damage during ischemia/reperfusion（I/R）. IP elicits vasodilation following ischemia-perfusion in the microvasculature；however, there are few precise data concerning the rate of blood flow following IP-mediated I/R in tissues. Nitric oxide（NO）plays an important role in vasodilation；however, the type of nitric oxide synthase（NOS）expression that influences IP has not yet been determined. Therefore, the present study aimed to determine the effect of IP on renal hemodynamics during I/R in rat renal I/R models and analyze NOS expression in IP and IP-mediated reperfused kidneys. 
Methods：Renal hemodynamics, function and tissue NOS-enzyme expression were estimated in the following animal groups：Group1；bilateral renal ischemia for 30-min and reperfusion group, Group 2：40-min ischemia group, Group 3；5-min ischemia（IP）prior to 30-min ischemia, Group 4；40-min ischemia with IP, and sham group.
Results：IP had beneficial effects on renal hemodynamics and function. We found that renal hemodynamics and glomerular filtration rate were significantly increased in IP-mediated kidneys. Western blotting analysis revealed increased expression levels of NOS-3 and NOS-2 in IP-mediated kidneys. 
Conclusion：These results suggest that IP-induced constitutive NOS may elicit a vasodilating effect in ischemia-reperfused rat kidneys., ＊ Ei-ichi Tsuji & Toshihisa Ogawa Contributed equally to this work},
 pages = {151--159},
 title = {Beneficial Effects of Ischemic Preconditioning on Renal Hemodynamics in Ischemia-reperfused Rat Kidneys：Role of Two Nitric Oxide Synthase Isozymes in Ischemic Preconditioned Kidneys},
 volume = {48},
 year = {2021}
}