{"created":"2023-06-20T14:29:13.395340+00:00","id":5255,"links":{},"metadata":{"_buckets":{"deposit":"2fd353d2-0ae8-409c-9f4a-2ce5473b02f4"},"_deposit":{"created_by":14,"id":"5255","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"5255"},"status":"published"},"_oai":{"id":"oai:dmu.repo.nii.ac.jp:00005255","sets":["223:233"]},"author_link":["47014","47010","47011","47012","47013"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"166","bibliographicPageStart":"157","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"Dokkyo Medical Journal"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"　Phosphatidylethanolamine (PE) and cholesterol are major lipid components of mammalian cell membranes. The rate limiting enzymes for the synthesis of PE and cholesterol are CTP: phosphoethanolamine cytidylyltransferase (Pcyt2) and 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), respectively. The transcription and activity of these enzymes in NIH3T3 cells are suppressed by fetal bovine serum (FBS) and hydroxylated cholesterol (oxysterols) in similar dose-dependent manners. These results suggested that the intrinsic oxysterol concentration in cells is important for maintaining membrane lipid components, leading us to investigate the regulation of oxysterol-producing enzyme activity in cells. Using NIH3T3 cells, we found that of all oxysterol-producing enzymes, only the 24S-hydroxycholesterol producing enzyme cytochrome P450 46A1 (Cyp46A1) is expressed. Transcription of Cyp46A1 is suppressed by FBS, and we identified insulin-like growth factor II (IGF-II) in FBS as the factor suppressing Cyp46A1 transcription. The oxysterol level in NIH3T3 cells is also suppressed by IGF-II. These results suggested that the classical hormone IGF-II regulates the oxysterol content in cells and transduces cellular signals.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Dokkyo Medical Society"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.51040/dkmj.2022-018","subitem_relation_type_select":"DOI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2436-522X","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_24":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Biochemistry, Dokkyo Medical University Tochigi Japan"},{"subitem_text_value":"Department of Biochemistry, Dokkyo Medical University Tochigi Japan"},{"subitem_text_value":"Department of Biochemistry, Dokkyo Medical University Tochigi Japan"},{"subitem_text_value":"Department of Biochemistry, Dokkyo Medical University Tochigi Japan"},{"subitem_text_value":"Department of Biochemistry, Dokkyo Medical University Tochigi Japan"}]},"item_10001_text_33":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_text_value":"Review Article"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ando, Hiromi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shinohara, Yasutake ","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Arai, Maki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Satoko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugimoto, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-12-26"}],"displaytype":"detail","filename":"DKMJ-1-3-2.pdf","filesize":[{"value":"970.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"DKMJ-1-3-Ando-Fulltext","url":"https://dmu.repo.nii.ac.jp/record/5255/files/DKMJ-1-3-2.pdf"},"version_id":"cc44019c-0b44-4aef-859b-6323df0d783d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Oxysterols","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphatidylethanolamine","subitem_subject_scheme":"Other"},{"subitem_subject":"Cholesterol","subitem_subject_scheme":"Other"},{"subitem_subject":"Pcyt2","subitem_subject_scheme":"Other"},{"subitem_subject":"Hmgcr","subitem_subject_scheme":"Other"},{"subitem_subject":"25-Hydroxycholesterol","subitem_subject_scheme":"Other"},{"subitem_subject":"Cytochrome P450 46A1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Regulation of Glycerophospholipid and Cholesterol Synthesis in Cells by Hydroxylated Cholesterols (Oxysterols)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Regulation of Glycerophospholipid and Cholesterol Synthesis in Cells by Hydroxylated Cholesterols (Oxysterols)"}]},"item_type_id":"10001","owner":"14","path":["233"],"publish_date":"2022-12-26","publish_status":"0","recid":"5255","relation_version_is_last":true,"title":["Regulation of Glycerophospholipid and Cholesterol Synthesis in Cells by Hydroxylated Cholesterols (Oxysterols)"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-06-20T14:44:54.116345+00:00"}