{"_buckets": {"deposit": "a1059656-64e7-4631-a628-ca25639861ce"}, "_deposit": {"created_by": 14, "id": "5256", "owners": [14], "pid": {"revision_id": 0, "type": "depid", "value": "5256"}, "status": "published"}, "_oai": {"id": "oai:dmu.repo.nii.ac.jp:00005256", "sets": ["233"]}, "author_link": ["47015", "238"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2022-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "175", "bibliographicPageStart": "167", "bibliographicVolumeNumber": "1", "bibliographic_titles": [{"bibliographic_title": "Dokkyo Medical Journal"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "　α-Taxilin is a member of the taxilin family, a binding partner of the syntaxin family involved in intracellular vesicle traffic. The taxilin family members share a long coiled-coil structure, which is homologous to that of Uso1, a yeast homologue of p115. It has been revealed that a parallel homodimerization of p115, an elongated polypeptide, is required for tethering of the transport vesicles to the acceptor membrane. In this study we examined whether α-taxilin has similar biochemical properties to p115. Gel filtration and sedimentation analyses suggest that α-taxilin is an elongated polypeptide containing a long coiled-coil structure. A pull-down assay revealed that His6-α-taxilin binds to GST-α-taxilin, and not to GST in dose-dependent and saturable manners. α-Taxilin formed a parallel homodimer through at least two independent regions. Together, α-taxilin may be involved in tethering of the transport vesicles to the acceptor membranes in a similar way to p115.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Dokkyo Medical Society"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.51040/dkmj.2022-002", "subitem_relation_type_select": "DOI"}}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2436-522X", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_text_24": {"attribute_name": "著者所属", "attribute_value_mlt": [{"subitem_text_value": "Department of Molecular and Cell Biology, Graduate School of Medicine, Dokkyo Medical University, Tochigi, Japan"}, {"subitem_text_value": "Department of Molecular and Cell Biology, Graduate School of Medicine, Dokkyo Medical University, Tochigi, Japan"}]}, "item_10001_text_33": {"attribute_name": "記事種別", "attribute_value_mlt": [{"subitem_text_value": "Original"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Higashi, Satoru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "47015", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shirataki, Hiromichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "238", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-12-26"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "DKMJ-1-3-3.pdf", "filesize": [{"value": "546.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 546100.0, "url": {"label": "DKMJ-1-3-Higashi-Fulltext", "url": "https://dmu.repo.nii.ac.jp/record/5256/files/DKMJ-1-3-3.pdf"}, "version_id": "06d80204-3033-433a-8519-120613eb8c3b"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "α-taxilin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "intracellular vesicle traffic", "subitem_subject_scheme": "Other"}, {"subitem_subject": "SNARE", "subitem_subject_scheme": "Other"}, {"subitem_subject": "syntaxin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Physicochemical Properties of α-Taxilin as a Putative Tethering Factor", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Physicochemical Properties of α-Taxilin as a Putative Tethering Factor"}]}, "item_type_id": "10001", "owner": "14", "path": ["233"], "permalink_uri": "https://dmu.repo.nii.ac.jp/records/5256", "pubdate": {"attribute_name": "公開日", "attribute_value": "2022-12-26"}, "publish_date": "2022-12-26", "publish_status": "0", "recid": "5256", "relation": {}, "relation_version_is_last": true, "title": ["Physicochemical Properties of α-Taxilin as a Putative Tethering Factor"], "weko_shared_id": -1}