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Physicochemical Properties of α-Taxilin as a Putative Tethering Factor
https://dmu.repo.nii.ac.jp/records/5256
https://dmu.repo.nii.ac.jp/records/5256d8ee8a96-d01e-453e-8ce0-56cc90d21760
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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タイトル | ||||||
タイトル | Physicochemical Properties of α-Taxilin as a Putative Tethering Factor | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | α-taxilin | |||||
キーワード | ||||||
主題 | intracellular vesicle traffic | |||||
キーワード | ||||||
主題 | SNARE | |||||
キーワード | ||||||
主題 | syntaxin | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Higashi, Satoru
× Higashi, Satoru× Shirataki, Hiromichi |
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著者所属 | ||||||
Department of Molecular and Cell Biology, Graduate School of Medicine, Dokkyo Medical University, Tochigi, Japan | ||||||
著者所属 | ||||||
Department of Molecular and Cell Biology, Graduate School of Medicine, Dokkyo Medical University, Tochigi, Japan | ||||||
書誌情報 |
Dokkyo Medical Journal 巻 1, 号 3, p. 167-175, 発行日 2022-09 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | α-Taxilin is a member of the taxilin family, a binding partner of the syntaxin family involved in intracellular vesicle traffic. The taxilin family members share a long coiled-coil structure, which is homologous to that of Uso1, a yeast homologue of p115. It has been revealed that a parallel homodimerization of p115, an elongated polypeptide, is required for tethering of the transport vesicles to the acceptor membrane. In this study we examined whether α-taxilin has similar biochemical properties to p115. Gel filtration and sedimentation analyses suggest that α-taxilin is an elongated polypeptide containing a long coiled-coil structure. A pull-down assay revealed that His6-α-taxilin binds to GST-α-taxilin, and not to GST in dose-dependent and saturable manners. α-Taxilin formed a parallel homodimer through at least two independent regions. Together, α-taxilin may be involved in tethering of the transport vesicles to the acceptor membranes in a similar way to p115. | |||||
記事種別 | ||||||
Original | ||||||
出版者 | ||||||
出版者 | Dokkyo Medical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2436-522X | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.51040/dkmj.2022-002 |