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  1. Dokkyo Journal of Medical Sciences
  2. 40(1) 2013

Validation of Pyrosequencing for the Analysis of KRAS Mutations in Colorectal Cancer

https://dmu.repo.nii.ac.jp/records/1085
https://dmu.repo.nii.ac.jp/records/1085
0e88464a-6def-4503-9310-3b639cd9c4d5
名前 / ファイル ライセンス アクション
KJ00008418079.pdf KJ00008418079.pdf (71.9 kB)
Item type [ELS]学術雑誌論文 / Journal Article(1)
公開日 2017-05-24
タイトル
タイトル Validation of Pyrosequencing for the Analysis of KRAS Mutations in Colorectal Cancer
言語 en
言語
言語 eng
キーワード
言語 en
主題 KRAS mutation
キーワード
言語 en
主題 pyrosequencing
キーワード
言語 en
主題 colorectal cancer
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
雑誌書誌ID
収録物識別子 AA00629581
論文名よみ
タイトル Validation of Pyrosequencing for the Analysis of KRAS Mutations in Colorectal Cancer
著者 Otake, Yosuke

× Otake, Yosuke

en Otake, Yosuke

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Fujimori, Takahiro

× Fujimori, Takahiro

en Fujimori, Takahiro

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Akimoto, Naohiko

× Akimoto, Naohiko

en Akimoto, Naohiko

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Ikematsu, Hiroaki

× Ikematsu, Hiroaki

en Ikematsu, Hiroaki

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Okamoto, Yosuke

× Okamoto, Yosuke

en Okamoto, Yosuke

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Yamaguchi, Takeshi

× Yamaguchi, Takeshi

en Yamaguchi, Takeshi

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Ichikawa, Kazuhito

× Ichikawa, Kazuhito

en Ichikawa, Kazuhito

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Tomita, Shigeki

× Tomita, Shigeki

en Tomita, Shigeki

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Saito, Yutaka

× Saito, Yutaka

en Saito, Yutaka

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著者所属(英)
en
Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine : Endoscopy Division, National Cancer Center Hospital
著者所属(英)
en
Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine
著者所属(英)
en
Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine : Division of Gastroenterology, Department of Internal Medicine, Nippon Medical School
著者所属(英)
en
Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine : Department of Gastorointestinal Oncology and Endoscopy, National Cancer Center Hospital
著者所属(英)
en
Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine : Division of Gastroenterology and Hepatology, Department of Internal Medicine, Toho University, Omori Medical Center
著者所属(英)
en
Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine : Department of Surgery 1, Dokkyo Medical University School of Medicine
著者所属(英)
en
Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine
著者所属(英)
en
Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine
著者所属(英)
en
Endoscopy Division, National Cancer Center Hospital
記事種別(英)
内容記述タイプ Other
内容記述 Original
抄録(英)
内容記述タイプ Other
内容記述 The use of antibodies against epidermal growth factor receptor( EGFR) in conjunction with conventionalchemotherapy for metastatic colorectal cancer (CRC) in patients with KRAS wild-type tumors has beenproven to be efficacious. Recently, KRAS testing prior to anti-EGFR therapy has become mandatory formetastatic CRC patients. Although newly developed pyrosequencing is expected to be one of the highthroughput procedures detecting such mutations, the accuracy of the procedure has not been well evaluated.In the present study, we aimed to validate the accuracy, especially the potential for a false-negative result,in detecting KRAS mutations by pyrosequencing using cultured tumor cells. DNA extracted from culturedìNOZî gallbladder cancer cells( known to contain KRAS mutation G12V) at concentrations of 1%, 5%, 10%, and 25%, as well as 2 DNA samples extracted from a resected CRC specimen( known to contain anotherKRAS mutation, G12C) at concentrations of 5% and 25%, were prepared. We analyzed KRAS mutationalstatus and nonexistent and/or nonfunctional mutations of these 6 samples using pyrosequencing. TheKRAS mutation detection rates in the 4 NOZ samples( 1%, 5%, 10%, and 25%) were 0.37%, 2.79%, 5.28%,and 13.85%, respectively. Some artifacts of KRAS mutations unlikely to be present were detected in 1%samples of NOZ at a rate similar to that of the G12V mutation( G12C, 0.29%;G13C, 0.42%). Although theKRAS mutation G12C was detected at rates of 1.26% and 6.49% in samples with 5% and 25% DNA extractedfrom resected CRC specimen, respectively, no other type of KRAS mutation was detected in suchsamples. Pyrosequencing could not detect KRAS mutations correctly in the sample containing 1% DNA.This might cause false negatives. A sample mutated DNA concentration of at least 5% was necessary forprecise analyses by this procedure.
書誌情報 Dokkyo journal of medical sciences

巻 40, 号 1, p. 55-59, 発行日 2013-03-25
ISSN
収録物識別子タイプ ISSN
収録物識別子 03855023
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