| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-05-24 |
| タイトル |
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タイトル |
Validation of Pyrosequencing for the Analysis of KRAS Mutations in Colorectal Cancer |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
|
主題 |
KRAS mutation |
| キーワード |
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言語 |
en |
|
主題 |
pyrosequencing |
| キーワード |
|
|
言語 |
en |
|
主題 |
colorectal cancer |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 雑誌書誌ID |
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収録物識別子 |
AA00629581 |
| 論文名よみ |
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タイトル |
Validation of Pyrosequencing for the Analysis of KRAS Mutations in Colorectal Cancer |
| 著者 |
Otake, Yosuke
Fujimori, Takahiro
Akimoto, Naohiko
Ikematsu, Hiroaki
Okamoto, Yosuke
Yamaguchi, Takeshi
Ichikawa, Kazuhito
Tomita, Shigeki
Saito, Yutaka
|
| 著者所属(英) |
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en |
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Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine : Endoscopy Division, National Cancer Center Hospital |
| 著者所属(英) |
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en |
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Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine |
| 著者所属(英) |
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en |
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Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine : Division of Gastroenterology, Department of Internal Medicine, Nippon Medical School |
| 著者所属(英) |
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en |
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Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine : Department of Gastorointestinal Oncology and Endoscopy, National Cancer Center Hospital |
| 著者所属(英) |
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en |
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Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine : Division of Gastroenterology and Hepatology, Department of Internal Medicine, Toho University, Omori Medical Center |
| 著者所属(英) |
|
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en |
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Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine : Department of Surgery 1, Dokkyo Medical University School of Medicine |
| 著者所属(英) |
|
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en |
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Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine |
| 著者所属(英) |
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en |
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Department of Surgical and Molecular Pathology, Dokkyo Medical University School of Medicine |
| 著者所属(英) |
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en |
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Endoscopy Division, National Cancer Center Hospital |
| 記事種別(英) |
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内容記述タイプ |
Other |
|
内容記述 |
Original |
| 抄録(英) |
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内容記述タイプ |
Other |
|
内容記述 |
The use of antibodies against epidermal growth factor receptor( EGFR) in conjunction with conventionalchemotherapy for metastatic colorectal cancer (CRC) in patients with KRAS wild-type tumors has beenproven to be efficacious. Recently, KRAS testing prior to anti-EGFR therapy has become mandatory formetastatic CRC patients. Although newly developed pyrosequencing is expected to be one of the highthroughput procedures detecting such mutations, the accuracy of the procedure has not been well evaluated.In the present study, we aimed to validate the accuracy, especially the potential for a false-negative result,in detecting KRAS mutations by pyrosequencing using cultured tumor cells. DNA extracted from culturedìNOZî gallbladder cancer cells( known to contain KRAS mutation G12V) at concentrations of 1%, 5%, 10%, and 25%, as well as 2 DNA samples extracted from a resected CRC specimen( known to contain anotherKRAS mutation, G12C) at concentrations of 5% and 25%, were prepared. We analyzed KRAS mutationalstatus and nonexistent and/or nonfunctional mutations of these 6 samples using pyrosequencing. TheKRAS mutation detection rates in the 4 NOZ samples( 1%, 5%, 10%, and 25%) were 0.37%, 2.79%, 5.28%,and 13.85%, respectively. Some artifacts of KRAS mutations unlikely to be present were detected in 1%samples of NOZ at a rate similar to that of the G12V mutation( G12C, 0.29%;G13C, 0.42%). Although theKRAS mutation G12C was detected at rates of 1.26% and 6.49% in samples with 5% and 25% DNA extractedfrom resected CRC specimen, respectively, no other type of KRAS mutation was detected in suchsamples. Pyrosequencing could not detect KRAS mutations correctly in the sample containing 1% DNA.This might cause false negatives. A sample mutated DNA concentration of at least 5% was necessary forprecise analyses by this procedure. |
| 書誌情報 |
Dokkyo journal of medical sciences
巻 40,
号 1,
p. 55-59,
発行日 2013-03-25
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
03855023 |
KJ00008418079.pdf (71.9 kB)
