| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-05-24 |
| タイトル |
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タイトル |
Identification of Carnitine Transporter CT1 Binding Protein Lin-7 in Nervous System |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
|
主題 |
Carnitine |
| キーワード |
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言語 |
en |
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主題 |
Transporter |
| キーワード |
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言語 |
en |
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主題 |
Pdz |
| キーワード |
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言語 |
en |
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主題 |
Yeast Two-hybrid Assay |
| キーワード |
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言語 |
en |
|
主題 |
Slc22 |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 雑誌書誌ID |
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収録物識別子 |
AA00629581 |
| 論文名よみ |
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タイトル |
Identification of Carnitine Transporter CT1 Binding Protein Lin-7 in Nervous System |
| 著者 |
Hanada, Kenji
Nakata, Takahiro
Ouchi, Motoshi
Ohtsubo, Misaki
Isono, Makoto
Morita, Asuka
Okamoto, Kazuhisa
Otani, Naoyuki
Kobayashi, Yasushi
Anzai, Naohiko
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| 著者所属(英) |
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en |
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Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine |
| 著者所属(英) |
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en |
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Department Of Anatomy And Cell Biology, Tokoha University:Department Of Health Science, Ishikawa Prefectural Nursing University |
| 著者所属(英) |
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en |
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Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine:Department Of Health Science, Ishikawa Prefectural Nursing University |
| 著者所属(英) |
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en |
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Department Of Health Science, Ishikawa Prefectural Nursing University:Department Of Health Science, Ishikawa Prefectural Nursing University |
| 著者所属(英) |
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en |
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Department Of Anatomy And Neurobiology, National Defense Medical College:Department Of Health Science, Ishikawa Prefectural Nursing University |
| 著者所属(英) |
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en |
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Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine:Department Of Health Science, Ishikawa Prefectural Nursing University |
| 著者所属(英) |
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en |
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Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine:Department Of Health Science, Ishikawa Prefectural Nursing University |
| 著者所属(英) |
|
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en |
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Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine:Department Of Health Science, Ishikawa Prefectural Nursing University |
| 著者所属(英) |
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en |
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Department Of Anatomy And Neurobiology, National Defense Medical College:Department Of Health Science, Ishikawa Prefectural Nursing University |
| 著者所属(英) |
|
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en |
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Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine:Department Of Health Science, Ishikawa Prefectural Nursing University |
| 記事種別(英) |
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内容記述タイプ |
Other |
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内容記述 |
Original |
| 抄録(英) |
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内容記述タイプ |
Other |
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内容記述 |
_L-Carnitine is an essential component of mitochondrial fatty acid b-oxidation in the muscle and may control the acetyl moiety levels in the brain for acetylcholine synthesis. Carnitine transporter 1(CT1)is the high affinity _L-carnitine transporter whose localization was observed in the kidney, testis, liver, skeletal muscle and brain. To clarify the molecular mechanism of carnitine transport, we sought to find the interacting protein that may be related to the transport function of CT1. Using the intracellular C-terminal region of rat CT1 containing PDZ(PSD95/DLG/ZO-1)motif as bait, we performed the yeast two-hybrid screening against rat brain cDNA library. Thirty two positive clones were obtained from the 2.7×10^7 clones screened. One of them was PDZ domain-containing protein Lin-7. We found that Lin-7 interacts specifically with C-termini of CT1:deletion and mutation of the CT1 C-terminal PDZ-motif abolished the interaction with Lin-7 in the yeast two-hybrid assay. In addition, a PDZ domain within Lin-7 associates with the CT1 C-terminal. The association of CT1 with Lin-7 enhanced _L-carnitine transport activities in HEK293 cells although there is no statistical significance. Coexpression of Lin-7 and CT1 is identified in motor neurons of the spinal cord ventral horn together with Lin-2, a binding partner of Lin-7 known to assemble proteins involved in synaptic vesicle exocytosis and synaptic junctions. Therefore, Lin-7 interacts with CT1 and may regulate their subcellular distribution or function in central nervous system. |
| 書誌情報 |
Dokkyo journal of medical sciences
巻 43,
号 1,
p. 31-38,
発行日 2016-03-25
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
03855023 |
KJ00010141603.pdf (273.3 kB)
