| Item type |
[ELS]学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-05-24 |
| タイトル |
|
|
タイトル |
Effects of Cytokines and Immunosuppressants on the Production of Serum Amyloid A Protein and C-reactive Protein in HepG2 Cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題 |
C-reactive protein |
| キーワード |
|
|
言語 |
en |
|
主題 |
serum amyloid A protein |
| キーワード |
|
|
言語 |
en |
|
主題 |
cytokines |
| キーワード |
|
|
言語 |
en |
|
主題 |
HepG2 cell |
| キーワード |
|
|
言語 |
en |
|
主題 |
Immunosuppressants |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 雑誌書誌ID |
|
|
収録物識別子 |
AA00629581 |
| 論文名よみ |
|
|
タイトル |
Effects of Cytokines and Immunosuppressants on the Production of Serum Amyloid A Protein and C-reactive Protein in HepG2 Cells |
| 著者 |
Shimetani, Naoto
Suzuki, Midori
Shimetani, Keiko
Nishikawa, Takashi
Mori, Mikio
|
| 著者所属(英) |
|
|
|
en |
|
|
Department of Clinical Laboratory, Koshigaya Hospital, Dokkyo University School of Medicine |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Clinical Laboratory, Koshigaya Hospital, Dokkyo University School of Medicine |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Clinical Pathology, Kitasato University School of Medicine |
| 著者所属(英) |
|
|
|
en |
|
|
Division of Medico-chemical Analysis, Kyoritsu College of Pharmacy |
| 著者所属(英) |
|
|
|
en |
|
|
Department of Clinical Laboratory, Koshigaya Hospital, Dokkyo University School of Medicine |
| 記事種別(英) |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Original |
| 抄録(英) |
|
|
内容記述タイプ |
Other |
|
内容記述 |
C-reactive protein (CRP) and serum amyloid A protein (SAA) are acute- phase proteins produced by the liver in response to inflammatory cytokines. The concentrations of these proteins in serum vary in parallel in most pathological conditions, but sometimes vary independently. CRP and SAA were determined in HepG2 cell culture medium supplemented with five immunosuppressants (corticosteroid, gusperimus hydrochloride, cyclosporin A, mizoribine and tacrolimus hydrate), with or without interleukin-1β(IL-1β) and interleukin-6 (IL-6). We also examined the effects of the immunosuppressants on the production of cytokines and changes in CRP and SAA production in HepG2 cells stimulated with the culture fluid from lipopolysaccharide (LPS) - treated monocytes. In HepG2 cells, production of CRP and SAA was greatly affected by IL-6 and IL-β, respectively. Prednisolone (PSL) suppressed CRP production, while it enhanced SAA production. The other four immunosuppressants did not affect CRP production, but inhibited SAA production. PSL significantly inhibited cytokine production in monocytes, while the other immunosuppressants enhanced it. In HepG2 cells incubated with the culture fluid from LPS-stimulated monocytes, CRP production was suppressed, while SAA production was enhanced. PSL suppressed CRP production in HepG2 cells by inhibiting IL-6 production in monocytes, whereas PSL increased SAA production through a direct action on the hepatoma cells. In contrast, the other immunosuppressive agents enhanced IL-β production in monocytes. The agents induced SAA production in the HepG2 cells but did not affect CRP production. |
| 書誌情報 |
Dokkyo journal of medical sciences
巻 30,
号 2,
p. 131-139,
発行日 2003-07-25
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03855023 |
KJ00000010468.pdf (1.1 MB)
