| Item type |
学術雑誌論文 / Journal Article(1) |
| タイトル |
|
|
タイトル |
Endocrine Secretory Granule Structures Form in the Presence of Granule-content Substances under REST-knockout and PROX1-expressing Conditions |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
endocrine secretory granule |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
PROX1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
REST |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
vasopressin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
calcitonin |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Ishii, Jun
Sato-Yazawa, Hanako
Kashiwagi, Korehito
Miyazawa, Tadasuke
Iwamoto, Masami
Miyata-Hiramatsu, Chie
Konno, Kodai
Miyata, Shunsuke
Kamimura, Tamotsu
Yazawa, Takuya
|
| 書誌情報 |
Dokkyo Medical Journal
巻 4,
号 5,
p. 378-388,
発行日 2025-10-25
|
| 記事種別 |
|
|
値 |
Original |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Endocrine secretory granules (ESGs) are intracellular organelles specific to endocrine/neuroendocrine cells, yet their precise mechanism of formation remains elusive. In our previous study, we successfully induced the formation of ESG structures in the nonendocrine lung cancer cell line H1299 by knocking out the REST gene, forcibly expressing PROX1, and inducing the expression of pro-opiomelanocortin (POMC), an ESG component. However, it is unclear whether ESG structures can form in the presence of intragranular contents other than POMC. In this study, we analyzed whether ESG structures form when calcitonin (calcitonin-related polypeptide α, CALCA variant 1) and vasopressin (arginine vasopressin, AVP), both of which are peptide hormones, are forcibly expressed in experimental systems described in previous studies. The forced expression of calcitonin in H1299 cells resulted in the formation of ESG structures under simultaneous REST knockout and forced PROX1 expression conditions. In cells that express vasopressin, ESG structures form under REST knockout conditions. Moreover, PROX1 overexpression led to the formation of a greater number of ESG structures. These results suggest that ESG structures form after the forced expression of any granulecontaining substance in H1299 cells under REST knockout and forced PROX1 expression conditions. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Dokkyo Medical Society |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2436-522X |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12941861 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.51040/dkmj.2025-007 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
DKMJ-4-5-4.pdf (1.2 MB)
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