ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "1491b1bd-30dd-4e78-8a4b-a3499ad4886e"}, "_deposit": {"created_by": 14, "id": "2100", "owners": [14], "pid": {"revision_id": 0, "type": "depid", "value": "2100"}, "status": "published"}, "_oai": {"id": "oai:dmu.repo.nii.ac.jp:00002100", "sets": ["178"]}, "author_link": ["10198", "10199", "6444"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-03-25", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "7", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "46", "bibliographic_titles": [{"bibliographic_title": "Dokkyo Journal of Medical Sciences"}]}]}, "item_10001_description_34": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "Background:Neurons containing enkephalin(ENK)are distributed at high concentrations in the superficial dorsal horn(SDH)of the spinal cord, where they play an important role in the modulation of nociceptive information. In addition to ENK, the SDH exhibits high expression levels of the NR2B subunit of the N-methyl-d-aspartate(NMDA)receptor and large-conductance calcium-activated potassium(BK)channels. In the present behavioral experiments, we investigated the effects of the BK channel antagonist charybdotoxin(CTX)and the NR2B subunit antagonist ifenprodil(IFN)on a nociceptive behavior in peripheral-nerve-injured mice.\nMethods:The experiments were performed in 6- to 8-week-old male ICR mice. Partial sciatic nerve ligation(PSL)was performed as described previously(Seltzer model). Mechanical allodynia was assessed by stimulation with von Frey filaments. On postsurgical day 7, the effects of CTX and IFN on mechanical allodynia were analyzed. Additionally, to test the possibility that the actions of CTX and IFN are mediated by an altered release of ENK, we investigated the effect of the selective μ- and δ-opioid receptor antagonists naloxone(NAL)and naltrindole(NTL), respectively. CTX(1 pmol/10μL per mouse), IFN(50 nmol/10μL per mouse), and saline as a control(10μL per mouse)were intrathecally injected. Additionally, NAL(5 mg/kg)and NTL(5 mg/kg)were intraperitoneally administered. All behavioral experiments were performed in a double-blind fashion.\nResults:PSL significantly increased the occurrence of the withdrawal reflex to von Frey stimuli, indicating the development of mechanical allodynia. CTX significantly reduced the occurrence of the withdrawal reflex compared to the saline group. Additionally, both NAL and NTL attenuated the analgesic effect of CTX. Intrathecal IFN significantly reduced the occurrence of the withdrawal reflex, which was also reduced by NAL and NTL.\nConclusion:The obtained behavioral observations suggest that the NMDA receptors and BK channels might inhibit the release of ENK in the SDH, which is speculated to be involved in the chronic pain state induced by peripheral nerve injury.\n\n", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "獨協医学会"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03855023  ", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_text_25": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Physiology \u0026 Biological Information, Dokkyo Medical University  Kitakobayashi 880, Mibu, Tochigi 321-0293, JAPAN"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Physiology \u0026 Biological Information, Dokkyo Medical University  Kitakobayashi 880, Mibu, Tochigi 321-0293, JAPAN"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Physiology \u0026 Biological Information, Dokkyo Medical University  Kitakobayashi 880, Mibu, Tochigi 321-0293, JAPAN"}]}, "item_10001_text_33": {"attribute_name": "記事種別", "attribute_value_mlt": [{"subitem_text_value": "Original"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kobayashi, Shunsaku", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6444", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Shiho", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "10198", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Eiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "10199", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-04-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "DJMS-46-1-2.pdf", "filesize": [{"value": "191.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 191000.0, "url": {"label": "DJMS-46-1-Kobayashi-本文", "url": "https://dmu.repo.nii.ac.jp/record/2100/files/DJMS-46-1-2.pdf"}, "version_id": "f7c5560e-bdc9-4797-bf30-218e0a5efb5f"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "NMDA receptor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "BK channel", "subitem_subject_scheme": "Other"}, {"subitem_subject": "enke­phalinergic neuron", "subitem_subject_scheme": "Other"}, {"subitem_subject": "neuropathic pain", "subitem_subject_scheme": "Other"}, {"subitem_subject": "spinal dorsal horn", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "NMDA NR2B Subunit Antagonist May Attenuate Mechanical Allodynia by Increasing the Release of Enkephalin in the Spinal Dorsal Horn", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "NMDA NR2B Subunit Antagonist May Attenuate Mechanical Allodynia by Increasing the Release of Enkephalin in the Spinal Dorsal Horn"}]}, "item_type_id": "10001", "owner": "14", "path": ["178"], "permalink_uri": "https://dmu.repo.nii.ac.jp/records/2100", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-04-25"}, "publish_date": "2019-04-25", "publish_status": "0", "recid": "2100", "relation": {}, "relation_version_is_last": true, "title": ["NMDA NR2B Subunit Antagonist May Attenuate Mechanical Allodynia by Increasing the Release of Enkephalin in the Spinal Dorsal Horn"], "weko_shared_id": -1}
  1. Dokkyo Journal of Medical Sciences
  2. 46(1) 2019

NMDA NR2B Subunit Antagonist May Attenuate Mechanical Allodynia by Increasing the Release of Enkephalin in the Spinal Dorsal Horn

https://dmu.repo.nii.ac.jp/records/2100
https://dmu.repo.nii.ac.jp/records/2100
3da6b8ed-e418-4ca4-b4d8-a044136b50e6
名前 / ファイル ライセンス アクション
DJMS-46-1-2.pdf DJMS-46-1-Kobayashi-本文 (191.0 kB)
Item type 学術雑誌論文 / Journal Article(1)
タイトル
タイトル NMDA NR2B Subunit Antagonist May Attenuate Mechanical Allodynia by Increasing the Release of Enkephalin in the Spinal Dorsal Horn
言語
言語 eng
キーワード
主題 NMDA receptor
キーワード
主題 BK channel
キーワード
主題 enke­phalinergic neuron
キーワード
主題 neuropathic pain
キーワード
主題 spinal dorsal horn
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
著者 Kobayashi, Shunsaku

× Kobayashi, Shunsaku

WEKO 6444

en Kobayashi, Shunsaku

Search repository
Tanaka, Shiho

× Tanaka, Shiho

WEKO 10198

en Tanaka, Shiho

Search repository
Kato, Eiko

× Kato, Eiko

WEKO 10199

en Kato, Eiko

Search repository
著者所属(英)
en
Department of Physiology & Biological Information, Dokkyo Medical University Kitakobayashi 880, Mibu, Tochigi 321-0293, JAPAN
著者所属(英)
en
Department of Physiology & Biological Information, Dokkyo Medical University Kitakobayashi 880, Mibu, Tochigi 321-0293, JAPAN
著者所属(英)
en
Department of Physiology & Biological Information, Dokkyo Medical University Kitakobayashi 880, Mibu, Tochigi 321-0293, JAPAN
書誌情報 Dokkyo Journal of Medical Sciences

巻 46, 号 1, p. 1-7, 発行日 2019-03-25
要旨
内容記述タイプ Other
内容記述 Background:Neurons containing enkephalin(ENK)are distributed at high concentrations in the superficial dorsal horn(SDH)of the spinal cord, where they play an important role in the modulation of nociceptive information. In addition to ENK, the SDH exhibits high expression levels of the NR2B subunit of the N-methyl-d-aspartate(NMDA)receptor and large-conductance calcium-activated potassium(BK)channels. In the present behavioral experiments, we investigated the effects of the BK channel antagonist charybdotoxin(CTX)and the NR2B subunit antagonist ifenprodil(IFN)on a nociceptive behavior in peripheral-nerve-injured mice.
Methods:The experiments were performed in 6- to 8-week-old male ICR mice. Partial sciatic nerve ligation(PSL)was performed as described previously(Seltzer model). Mechanical allodynia was assessed by stimulation with von Frey filaments. On postsurgical day 7, the effects of CTX and IFN on mechanical allodynia were analyzed. Additionally, to test the possibility that the actions of CTX and IFN are mediated by an altered release of ENK, we investigated the effect of the selective μ- and δ-opioid receptor antagonists naloxone(NAL)and naltrindole(NTL), respectively. CTX(1 pmol/10μL per mouse), IFN(50 nmol/10μL per mouse), and saline as a control(10μL per mouse)were intrathecally injected. Additionally, NAL(5 mg/kg)and NTL(5 mg/kg)were intraperitoneally administered. All behavioral experiments were performed in a double-blind fashion.
Results:PSL significantly increased the occurrence of the withdrawal reflex to von Frey stimuli, indicating the development of mechanical allodynia. CTX significantly reduced the occurrence of the withdrawal reflex compared to the saline group. Additionally, both NAL and NTL attenuated the analgesic effect of CTX. Intrathecal IFN significantly reduced the occurrence of the withdrawal reflex, which was also reduced by NAL and NTL.
Conclusion:The obtained behavioral observations suggest that the NMDA receptors and BK channels might inhibit the release of ENK in the SDH, which is speculated to be involved in the chronic pain state induced by peripheral nerve injury.
記事種別
Original
出版者
出版者 獨協医学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 03855023
戻る
0
views
See details
Views

Versions

Ver.1 2023-06-20 15:35:04.942686
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3