{"_buckets": {"deposit": "a9b95f56-447a-4372-8412-3ca39e24f02d"}, "_deposit": {"created_by": 3, "id": "221", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "221"}, "status": "published"}, "_oai": {"id": "oai:dmu.repo.nii.ac.jp:00000221", "sets": ["29"]}, "author_link": ["785", "781", "786", "784", "782", "783"], "item_2_biblio_info_12": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-03-25", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "89", "bibliographicPageStart": "79", "bibliographicVolumeNumber": "31", "bibliographic_titles": [{"bibliographic_title": "Dokkyo journal of medical sciences"}]}]}, "item_2_description_10": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "食道粘膜傷害の発生機序と粘膜修復機転の解明は,臨床的に逆流性食道炎に代表されるような重要な課題であるにもかかわらず,そのメカニズムは十分検討されていない.我々は,これまで消化管の粘膜障害の再生機転を, cyclooxygenaze-2 COX-2)発現→prostaglandin (PG) 産生→hepatocyte growth factor(HGF)→修復という一連の過程を仮説として提唱してきた.そこで培養食道上皮細胞を用いてCOX-2, PG,HGFを測定して、消化管粘膜の代表的障害因子であるステロイドと非ステロイド系消炎鎮痛薬(Non-steroidal anti-inflammatory drugs ; 以下, NSAIDs)の食道修復に与える影響について検討した.培養扁平上皮細胞に欠損部を作成し修復速度を観察すると,細胞修復はステロイドやNSAIDsにより抑制された.これら抑制された上皮修復は外因性HGFにより回復した. PGの遊離に関しても各薬剤によりその遊離が阻害された. HGFの発現はステロイド, NSAIDsにより抑制された.これに対しステロイドはCOX-2の発現を抑制したが, NSAIDsはCOX-2の発現を抑制しなかった.これらの結果よりステロイドはCOX-2の発現を抑制し,またNSAIDsはPG遊離を抑制することにより培養食道細胞の修復が阻害されたものと考えられた.", "subitem_description_type": "Other"}]}, "item_2_description_11": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "BACKGROUNDS : Repeated wounding and healing at the esophago - gastric junction may be a cause of Barrett esophagus. Therefore, the mechanism of wound healing of gastric epithelial cells and esophageal cells may be important for the understanding Barrett esophagus development. We have previously shown that hepatocyte growth factor (HGF) plays a role in esophageal epithelial restitution, using in vitro cultured cells. We have also demonstrated that the source of HGF is esophageal fibroblasts and the prostaglandin E (PGE) induces HGF production by the fibroblasts. In the present study, we investigated the effect of dexamethasone and NSAID on fibroblast PGE and HGF production and on cell restitution in the round wound restitution model. METHODS : 1. Primary culture of esophageal epithelial cells and fibroblasts were prepared from rabbit esophagus, after enzyme digestion. 2. Restitution was assessed by a round wound restitution model of cultured cells. The monolayer was wounded with a custom-made scraper which produced a round wound of 1.0 mm in diameter. The sizes of denuded areas were measured over time, after incubation with or without dexamathasone and NSAIDs. 3. Total cellular RNA was extracted from the cells. TaqMan RT-PCR was performed to quantitate HGF and COX-2 mRNA expression. 4. PGE2 was measured by ELISA. RESULTS : 1. Dexamethasone, etodolac, sulindac sulfide, and NS 398 (NSAID) inhibited the restitution of co-culture of esophageal epithelial cells and fibroblasts, which was reversed by HGF. 2. Dexamethasone and NSAID inhibited the production of PGE2, as assessed by ELISA. 3. Dexamethasone inhibited the expression of COX-2 mRNA but NSAID did not. 5. Dexamethasone and NSAID both inhibited HGF mRNA expression. CONCLUSION : These results suggest a sequence of cell repair process in esophageal cells ; namely, COX-2--PG--HGF--Restitution. This sequence is inhibited by dexamethasone via inhibition of COX-2 expression, and by NSAID via inhibition of PG production.", "subitem_description_type": "Other"}]}, "item_2_description_15": {"attribute_name": "表示順", "attribute_value_mlt": [{"subitem_description": "12", "subitem_description_type": "Other"}]}, "item_2_description_16": {"attribute_name": "アクセション番号", "attribute_value_mlt": [{"subitem_description": "KJ00004091314", "subitem_description_type": "Other"}]}, "item_2_description_8": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_description": "原著", "subitem_description_type": "Other"}]}, "item_2_description_9": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_description": "Original", "subitem_description_type": "Other"}]}, "item_2_source_id_1": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00629581", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_19": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03855023", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_6": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "獨協医科大学越谷病院消化器内科"}, {"subitem_text_value": "獨協医科大学越谷病院消化器内科"}, {"subitem_text_value": "獨協医科大学越谷病院消化器内科"}]}, "item_2_text_7": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Depertment of Gastroenterology and Hepatology, Koshigaya Hospital, Dokkyo University School of Medicine"}, {"subitem_text_language": "en", "subitem_text_value": "Depertment of Gastroenterology and Hepatology, Koshigaya Hospital, Dokkyo University School of Medicine"}, {"subitem_text_language": "en", "subitem_text_value": "Depertment of Gastroenterology and Hepatology, Koshigaya Hospital, Dokkyo University School of Medicine"}]}, "item_2_title_3": {"attribute_name": "論文名よみ", "attribute_value_mlt": [{"subitem_title": "ショクドウ ネンマク サイボウ シュウフク ニオケル HGF(Hepatocyte Growth Factor) ノ ヤクワリ ト NSAIDs(Non-steroidal anti-inflammatory drugs) ノ サヨウ"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "高田, 博信"}, {"creatorName": "タカダ, ヒロノブ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "781", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "高橋, 盛男"}, {"creatorName": "タカハシ, モリオ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "782", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "岡, 茂樹"}, {"creatorName": "オカ, シゲキ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "783", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takada, Hironobu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "784", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takahashi, Morio", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "785", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oka, Shigeki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "786", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-05-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00004091314.pdf", "filesize": [{"value": "932.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 932000.0, "url": {"label": "KJ00004091314.pdf", "url": "https://dmu.repo.nii.ac.jp/record/221/files/KJ00004091314.pdf"}, "version_id": "7216b5f9-6950-49d4-8905-6593750a61f7"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "食道粘膜修復,", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ステロイド", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NSAIDs", "subitem_subject_scheme": "Other"}, {"subitem_subject": "COX-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "HGF", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Esophageal restitution", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Dexamethasone", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NSAIDs", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "COX-2", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "HGF", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "食道粘膜細胞修復におけるHGF(Hepatocyte Growth Factor)の役割とNSAIDs(Non-steroidal anti-inflammatory drugs)の作用", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "食道粘膜細胞修復におけるHGF(Hepatocyte Growth Factor)の役割とNSAIDs(Non-steroidal anti-inflammatory drugs)の作用"}, {"subitem_title": "Effect of Nsaid on Restitution of Esophageal Cells and Role of Hepatocyte Growth Factor", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "3", "path": ["29"], "permalink_uri": "https://dmu.repo.nii.ac.jp/records/221", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-05-24"}, "publish_date": "2017-05-24", "publish_status": "0", "recid": "221", "relation": {}, "relation_version_is_last": true, "title": ["食道粘膜細胞修復におけるHGF(Hepatocyte Growth Factor)の役割とNSAIDs(Non-steroidal anti-inflammatory drugs)の作用"], "weko_shared_id": 3}