{"_buckets": {"deposit": "6773bacd-393b-41bd-8583-5a3367f352a6"}, "_deposit": {"created_by": 3, "id": "294", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "294"}, "status": "published"}, "_oai": {"id": "oai:dmu.repo.nii.ac.jp:00000294", "sets": ["31"]}, "author_link": ["1183", "1186", "1184", "1187", "1182", "1188", "1185", "1181", "1189"], "item_2_biblio_info_12": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-10-25", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "223", "bibliographicPageStart": "217", "bibliographicVolumeNumber": "31", "bibliographic_titles": [{"bibliographic_title": "Dokkyo journal of medical sciences"}]}]}, "item_2_description_10": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "魚脂に含まれるω3多価不飽和脂肪酸(ω3 polyunsaturated fatty add : ω3 PUFA)であるイコサペント酸エチル(eicosapentaenoic acid : EPA)は潰瘍性大腸炎などに対して臨床効果があると報告されている.一方, EPAは胃潰瘍の予防に対して有効であるというin vivoの報告もある.そこで今回我々は胃上皮細胞の倍養系を用いて上皮細胞の修復に対するEPAの影響を検討した.胃粘膜上皮細胞はラット正常胃粘膜より分離した細胞株であるRGM-1を用いた.Prostaglandin(PG)は細胞上清をELISA法にて測定した.細胞数はWST-8法により測定した.細胞障害は上清のLDHを測定することにより評価した.上皮細胞の修復は,単層培養細胞にスクレーパーを用いて上皮細胞に円形欠損を作成し,時間をおって欠損面積を測定して,速く欠損面積が減少したものを修復促進と評価した.EPAをω6多価不飽和脂肪酸(ω6 polyunsaturated fatty acid : ω6 PUFA)のみを含む培養液に添加すると,上皮細胞修復は濃度依存的に促進した.EPAは,PGE_2の遊離を抑制した.EPAを添加しても生存細胞の数や障害に影響は認められなかった.以上の成績より,ω6 PUFAであるリノール酸のみで,ω3PUFAが存在しない培養液では,上皮修復に不利でありω3PUFAであるEPAを補うことにより修復は回復すると結論された.", "subitem_description_type": "Other"}]}, "item_2_description_11": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "BACKGROUND Epidemiological studies have shown that a high consumption of fish is associated with a low prevalence of peptic ulcers, which is still contraversial. The beneficial effect of eicosapentaenoic acid (EPA), which is abundant in fish oil, on gastric ulcers and on ulcerative colitis has been climically demonsrated. However, the mechanism of the anti-ulcer action of EPA is not known. In the present study, we investigated the effect of EPA on gastric restitution using in vitro culture restitution model. METHODS 1. Ras normal gastric epithelial cell line, RGM-1 was used. 2. Epithelial cell restitution was assessed by round wound restitution assay 3. PGE2 was measured by enzyme-linked immunosorbent assay (ELISA). 4. Assessment of cell damages were determined by WST-8 assay (modified MTT assay) and LDH release. RESULTS 1. EPA accelerated the restitution of PGM-1.2. EPA inhibited PGE2 release. 3. EPA did not influence the damage of RGM-1 damage, as assessed by WST-8 assay. 4. EPA did not have any effect on LDH release. CONCLUSION Addition of EPA accelerated the restitution of gastric epithelial cells, when n3 polyunsaturated fatty acid is deficient.", "subitem_description_type": "Other"}]}, "item_2_description_15": {"attribute_name": "表示順", "attribute_value_mlt": [{"subitem_description": "6", "subitem_description_type": "Other"}]}, "item_2_description_16": {"attribute_name": "アクセション番号", "attribute_value_mlt": [{"subitem_description": "KJ00004161777", "subitem_description_type": "Other"}]}, "item_2_description_8": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_description": "原著", "subitem_description_type": "Other"}]}, "item_2_description_9": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_description": "Original", "subitem_description_type": "Other"}]}, "item_2_source_id_1": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00629581", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_19": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03855023", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_6": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "獨協医科大学越谷病院消化器内科"}, {"subitem_text_value": "獨協医科大学越谷病院消化器内科"}, {"subitem_text_value": "獨協医科大学越谷病院消化器内科"}, {"subitem_text_value": "獨協医科大学越谷病院消化器内科"}, {"subitem_text_value": "医学総合研究所"}]}, "item_2_text_7": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Departments of Gastroenterology and Hepatology, Koshigaya Hospital, Dokkyo University School of Medicine"}, {"subitem_text_language": "en", "subitem_text_value": "Departments of Gastroenterology and Hepatology, Koshigaya Hospital, Dokkyo University School of Medicine"}, {"subitem_text_language": "en", "subitem_text_value": "Takada,Hiroshi"}, {"subitem_text_language": "en", "subitem_text_value": "Departments of Gastroenterology and Hepatology, Koshigaya Hospital, Dokkyo University School of Medicine /"}]}, "item_2_title_3": {"attribute_name": "論文名よみ", "attribute_value_mlt": [{"subitem_title": "ω3 タカ フホウワ シボウサン ノ イ ネンマク ジョウヒ サイボウ シュウフク ニ アタエル エイキョウ"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "高田, 博信"}, {"creatorName": "タカダ, ヒロノブ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "1181", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "高橋, 盛男"}, {"creatorName": "タカハシ, モリオ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "1182", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "高田, 洋"}, {"creatorName": "タカダ, ヒロシ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "1183", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "桑山, 肇"}, {"creatorName": "クワヤマ, ハジメ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "1184", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "相馬, 良一"}, {"creatorName": "ソウマ, リョウイチ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "1185", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takada, Hironobu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1186", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takahashi, Morio", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1187", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuwayama, Hajime", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1188", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Soma, Ryouichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1189", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-05-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00004161777.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1000000.0, "url": {"label": "KJ00004161777.pdf", "url": "https://dmu.repo.nii.ac.jp/record/294/files/KJ00004161777.pdf"}, "version_id": "7bb60f71-434b-46c2-9fb2-e70ef34feb12"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "ω3多価不飽和脂肪酸", "subitem_subject_scheme": "Other"}, {"subitem_subject": "eicosapentaenoic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "胃潰瘍", "subitem_subject_scheme": "Other"}, {"subitem_subject": "修復", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "ω3多価不飽和脂肪酸の胃粘膜上皮細胞修復に与える影響", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "ω3多価不飽和脂肪酸の胃粘膜上皮細胞修復に与える影響"}, {"subitem_title": "ω3 Polyunsaturated Fatty Acid and Ulcer Healing", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "3", "path": ["31"], "permalink_uri": "https://dmu.repo.nii.ac.jp/records/294", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-05-24"}, "publish_date": "2017-05-24", "publish_status": "0", "recid": "294", "relation": {}, "relation_version_is_last": true, "title": ["ω3多価不飽和脂肪酸の胃粘膜上皮細胞修復に与える影響"], "weko_shared_id": 3}