| Item type |
学術雑誌論文 / Journal Article(1) |
| タイトル |
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タイトル |
Effects of Peripheral Nerve Injury on the Kinetics of Electrically Evoked GABA Receptor-mediated Currents in GABAergic and Non-GABAergic Neurons of the Spinal Dorsal Horn |
| 言語 |
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言語 |
eng |
| キーワード |
|
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主題Scheme |
Other |
|
主題 |
neuropathic pain |
| キーワード |
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主題Scheme |
Other |
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主題 |
peripheral nerve injury |
| キーワード |
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主題Scheme |
Other |
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主題 |
GABA |
| キーワード |
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主題Scheme |
Other |
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主題 |
glutamate decarboxylase |
| キーワード |
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主題Scheme |
Other |
|
主題 |
green fluorescent protein |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Ajima, Takaaki
Yamaguchi, Shigeki
Takasusuki, Toshifumi
Kato, Eiko
Hori, Yuuichi
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| 著者所属(英) |
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en |
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Department of Anesthesiology, Dokkyo Medical University, Tochigi, Japan |
| 著者所属(英) |
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en |
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Department of Anesthesiology, Dokkyo Medical University, Tochigi, Japan |
| 著者所属(英) |
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en |
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Department of Anesthesiology, Dokkyo Medical University, Tochigi, Japan |
| 著者所属(英) |
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en |
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Department of Physiology, Dokkyo Medical University, Tochigi, Japan |
| 著者所属(英) |
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en |
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Department of Physiology, Dokkyo Medical University, Tochigi, Japan |
| 書誌情報 |
Dokkyo Medical Journal
巻 1,
号 2,
p. 99-107,
発行日 2022-06
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The neurotransmitters and neuromodulators implicated in the processing of sensory and nociceptive information, γ-aminobutyric acid (GABA) is of importance in the spinal dorsal horn (SDH) neurons. Decrease in GABAergic inhibitory synaptic transmission plays an important role in mechanisms of neuropathic pain (NeP). We hypothesized that functional and structural changes in GABA receptors of SDH may occur after peripheral injury (PNI). In the present study, using whole-cell patch-clamp recording, we characterized the GABA receptor-mediated currents (IGABA) and GABA receptor-mediated inhibitory postsynaptic currents (GABA-IPSCs) recorded from SDH neurons, and investigated the effects of the sciatic nerve ligation on these currents in the glutamate decarboxylase (GAD) 67 green fluorescent protein (GAD67-GFP) knock-in mice. Furthermore, using the quantitative real-time PCR analysis, we evaluated alterations of the expression level of the GABA receptor subunits by the sciatic nerve ligation. We observed changes in IGABA and GABA-IPSCs in both GFP-negative and positive neurons, and alterations of the expression level of the GABAA receptor subunits, in SDH after the sciatic nerve ligation. Our results suggest that influences on the function and structure of GABAergic neuron in SDH by PNI may play an important role in the mechanism of NeP. |
| 記事種別 |
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Original |
| 出版者 |
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出版者 |
Dokkyo Medical Society |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2436-5211 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.51040/dkmj.1.2_99 |
DKMJ-1-2-Ajima-Fulltext (322.3 kB)
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