{"_buckets": {"deposit": "2050d231-23b6-478b-86b3-04e7111a5554"}, "_deposit": {"created_by": 3, "id": "754", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "754"}, "status": "published"}, "_oai": {"id": "oai:dmu.repo.nii.ac.jp:00000754", "sets": ["49"]}, "author_link": ["3148", "3150", "3151", "3149"], "item_2_biblio_info_12": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2009-03-25", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "58", "bibliographicPageStart": "47", "bibliographicVolumeNumber": "36", "bibliographic_titles": [{"bibliographic_title": "Dokkyo journal of medical sciences"}]}]}, "item_2_description_10": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "SOX2 は SRY-related HMG box ファミリーに属する転写因子であり,SOX2の胃粘膜上皮細胞における発現は以前より報告されているが,胃特異的遺伝子発現へのSOX2の影響は十分には解明されていない.本研究では胃癌由来細胞株(MKN45細胞,AGS細胞)を用い,胃粘膜上皮細胞におけるTFF およびMUC の発現に対するSOX2の影響について検討を行った.両細胞株においてSOX2蛋白の発現をウエスタンブロット法で確認し,TFF(TFF1,TFF2,TFF3)およびMUC(MUC2,MUC5AC,MUC6)それぞれのレポーター遺伝子を用いた解析を行った.SOX2の過剰発現で影響を受けたのはTFF1とMUC6であり,TFF1発現は有意に低下,逆にMUC6発現は有意に増加することが認められた.リアルタイム定量的RT-PCR により,内因性のTFF1およびMUC6 mRNAもSOX2過剰発現により同様の効果を受けることを確認した.本研究の結果は,SOX2が消化管において胃粘膜に特異的に発現しているTFF(TFF1,TFF2)およびMUC(MUC5AC,MUC6)の発現を一様に誘導しているのではなく,異なった影響を与えていること,また,本来,消化管において腸粘膜に特異的に発現しているTFF3,MUC2の胃粘膜における発現の抑制に機能しているのではないことを示唆している.今後,他の様々な因子との相互作用を念頭に置いた上で,胃粘膜上皮におけるSOX2の機能をさらに解明していく必要があると考えられる.", "subitem_description_type": "Other"}]}, "item_2_description_11": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "Although previous studies showed the expression ofSOX2, a SRY-related HMG box family transcription factor,in gastric epithelial cells, little is known about the regulationof stomach-specific gene expression by SOX2. In this study,by using gastric cancer cell lines (MKN45 and AGS) as amodel system, we examined the effects of SOX2 on the expressionof trefoil factor family peptides( TFF1, TFF2, andTFF3) and mucin core proteins (MUC2, MUC5AC, andMUC6). Reporter gene analyses showed that forced overexpressionof SOX2 proteins significantly down-regulatesthe transcription of TFF1 and up-regulates the transcriptionof MUC6. TFF2 and MUC5AC transcription wasslightly repressed by SOX2. TFF3 and MUC2 transcriptionwas slightly up-regulated by SOX2 in MKN45 cells butslightly down-regulated in AGS cells. Real-time quantitativeRT-PCR also confirmed the down-regulation of endogenousTFF1 mRNA expression and up-regulation of MUC6mRNA expression by SOX2 overexpression. These resultssuggest that SOX2 differentially affects the expression ofstomach-specific TFFs (TFF1 and TFF2) and MUCs(MUC5AC and MUC6), and that SOX2 may not be a keyfactor for the repression of the expression of intestine-specificTFF (TFF3) and MUC(MUC2) in gastric epithelialcells.", "subitem_description_type": "Other"}]}, "item_2_description_15": {"attribute_name": "表示順", "attribute_value_mlt": [{"subitem_description": "8", "subitem_description_type": "Other"}]}, "item_2_description_16": {"attribute_name": "アクセション番号", "attribute_value_mlt": [{"subitem_description": "KJ00006353199", "subitem_description_type": "Other"}]}, "item_2_description_8": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_description": "原著", "subitem_description_type": "Other"}]}, "item_2_description_9": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_description": "Original", "subitem_description_type": "Other"}]}, "item_2_source_id_1": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00629581", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_19": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03855023", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_6": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "獨協医科大学内科学(消化器)"}, {"subitem_text_value": "獨協医科大学内科学(消化器)"}]}, "item_2_text_7": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Gastroenterology, Dokkyo Medical University School of Medicine"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Gastroenterology, Dokkyo Medical University School of Medicine"}]}, "item_2_title_3": {"attribute_name": "論文名よみ", "attribute_value_mlt": [{"subitem_title": "イ ネンマク ジョウヒ サイボウ ノ トレフォイル ファクター (TFF) オヨビ ムチンコア タンパク (MUC) ハツゲン ニ タイスル SOX2 ノ エイキョウ"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "鈴木, 真琴"}, {"creatorName": "スズキ, マコト", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "3148", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "島田, 忠人"}, {"creatorName": "シマダ, タダヒト", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "3149", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Makoto", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "3150", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimada, Tadahito", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "3151", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-05-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00006353199.pdf", "filesize": [{"value": "557.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 557600.0, "url": {"label": "KJ00006353199.pdf", "url": "https://dmu.repo.nii.ac.jp/record/754/files/KJ00006353199.pdf"}, "version_id": "e94b4902-f65c-421e-95a9-f81115e68575"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "胃粘膜上皮細胞", "subitem_subject_scheme": "Other"}, {"subitem_subject": "trefoil factor family", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ムチンコア蛋白", "subitem_subject_scheme": "Other"}, {"subitem_subject": "SOX2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gastric epithelial cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "trefoil factor family", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mucin core protein", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "SOX2", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "胃粘膜上皮細胞のトレフォイルファクター (TFF) およびムチンコア蛋白 (MUC) 発現に対するSOX2の影響", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "胃粘膜上皮細胞のトレフォイルファクター (TFF) およびムチンコア蛋白 (MUC) 発現に対するSOX2の影響"}, {"subitem_title": "SOX2 Down-regulates TFF1 Expression and UP-regulates MUC6 Expression in Gastric Epithelial Cells", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "3", "path": ["49"], "permalink_uri": "https://dmu.repo.nii.ac.jp/records/754", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-05-24"}, "publish_date": "2017-05-24", "publish_status": "0", "recid": "754", "relation": {}, "relation_version_is_last": true, "title": ["胃粘膜上皮細胞のトレフォイルファクター (TFF) およびムチンコア蛋白 (MUC) 発現に対するSOX2の影響"], "weko_shared_id": 3}