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In normal vascular endothelial cells, the insulin effect via the PI3K pathwayis thought to occur slightly earlier than that via the MAPK pathway. However, the direct effect of insulinon vascular rings isolated from an animal model has not been fully examined. The main purpose of studywas to investigate the potential vasodilation-effect of insulin on the isolated vascular rings of rats.Material and Methods:Descending aorta rings isolated from Wister rats were used in exo-vivo study.In study using culture cells, bovine aortic endothelial cells were used, and the effect of insulin on the productionof nitric oxide( NO) and the secretion of endothelin( ET)-1 was investigated. 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  1. Dokkyo Journal of Medical Sciences
  2. 37(1) 2010

Effect of Insulin on Aortic Rings Isolated from Wistar Rats

https://dmu.repo.nii.ac.jp/records/825
https://dmu.repo.nii.ac.jp/records/825
49684cef-2164-4fdd-a629-ef63942cc696
名前 / ファイル ライセンス アクション
KJ00006371060.pdf KJ00006371060.pdf (218.5 kB)
Item type [ELS]学術雑誌論文 / Journal Article(1)
公開日 2017-05-24
タイトル
言語 en
タイトル Effect of Insulin on Aortic Rings Isolated from Wistar Rats
言語
言語 eng
キーワード
言語 en
主題 insulin
キーワード
言語 en
主題 NO
キーワード
言語 en
主題 ET-1
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
雑誌書誌ID
収録物識別子 AA00629581
論文名よみ
タイトル Effect of Insulin on Aortic Rings Isolated from Wistar Rats
著者 Takebayashi, Kohzo

× Takebayashi, Kohzo

WEKO 3501

en Takebayashi, Kohzo

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Sohma, Ryouichi

× Sohma, Ryouichi

WEKO 3502

en Sohma, Ryouichi

Search repository
Naruse, Rika

× Naruse, Rika

WEKO 3503

en Naruse, Rika

Search repository
Aso, Yoshimasa

× Aso, Yoshimasa

WEKO 3504

en Aso, Yoshimasa

Search repository
Inukai, Toshihiko

× Inukai, Toshihiko

WEKO 3505

en Inukai, Toshihiko

Search repository
著者所属(日)
Department of Internal Medicine, Dokkyo Medical University Koshigaya Hospital
著者所属(日)
Joint Research Center, Dokkyo Medical University Koshigaya Hospital
著者所属(日)
Department of Internal Medicine, Dokkyo Medical University Koshigaya Hospital
著者所属(日)
Department of Internal Medicine, Dokkyo Medical University Koshigaya Hospital
著者所属(日)
Department of Internal Medicine, Dokkyo Medical University Koshigaya Hospital
記事種別(日)
内容記述タイプ Other
内容記述 原著
記事種別(英)
内容記述タイプ Other
内容記述 Original
抄録(英)
内容記述タイプ Other
内容記述 Background:Insulin increases nitric oxide (NO) production via the phosphatidylinositol 3-kinase(PI3K) pathway and endothelin( ET)-1 secretion via the mitogen-activated protein kinase( MAPK) pathwayin vascular endothelial cells. In normal vascular endothelial cells, the insulin effect via the PI3K pathwayis thought to occur slightly earlier than that via the MAPK pathway. However, the direct effect of insulinon vascular rings isolated from an animal model has not been fully examined. The main purpose of studywas to investigate the potential vasodilation-effect of insulin on the isolated vascular rings of rats.Material and Methods:Descending aorta rings isolated from Wister rats were used in exo-vivo study.In study using culture cells, bovine aortic endothelial cells were used, and the effect of insulin on the productionof nitric oxide( NO) and the secretion of endothelin( ET)-1 was investigated. NO production was evaluatingusing diaminofluorescein-2, a NO-specific dye.Results:Insulin (250 nM)-stimulated NO production evaluated using diaminofluorescein-2 (DAF-2) dependencyon the NO concentration in BAECs indicated a significant increase in NO compared with controls.Addition of 250 nM insulin also significantly increased ET-1 secretion and phosphorylation of Akt at Ser473and eNOS at Ser1177 (Ser1179 in bovine eNOS) in BAECs, compared with controls. Insulin treatmentshowed a slight tendency to increase phosphorylation of extracellular signal-regulated kinase (ERK) 1/2,but this change did not reach statistical significance. A cumulative insulin concentration of 3×10−6 M producedonly a small change in the diameter of aortic rings from Wister rats( 7.3±3.5% vs. 3.6±3.5% with 3×10−6 M saline;both n=3). Addition of acetylcholine to these rings caused dilatation of almost 100 %.Conclusion:Insulin increases production of NO and secretion of ET-1 in BAECs, but has little effect onaortic rings isolated from Wister rats.
書誌情報 Dokkyo journal of medical sciences

巻 37, 号 1, p. 9-14, 発行日 2010-03-25
ISSN
収録物識別子 03855023
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