{"_buckets": {"deposit": "a534f2c8-bc8a-4a65-9203-e2913d1e2126"}, "_deposit": {"created_by": 3, "id": "827", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "827"}, "status": "published"}, "_oai": {"id": "oai:dmu.repo.nii.ac.jp:00000827", "sets": ["53"]}, "author_link": ["3551", "3548", "3549", "3550"], "item_2_biblio_info_12": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-03-25", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "29", "bibliographicPageStart": "21", "bibliographicVolumeNumber": "37", "bibliographic_titles": [{"bibliographic_title": "Dokkyo journal of medical sciences"}]}]}, "item_2_description_10": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "All-trans retinoic acid(ATRA)は核内受容体であるretinoic acid receptor(RAR)のリガンドであり,消化管を含む多くの組織で重要な生理作用を有している.最近,臨床的なレチノイン酸の使用と炎症性腸疾患との関連が注目されているが,この問題に関する基礎的な検討は行われていない.今回我々は,大腸癌由来細胞株を用いて,炎症・免疫応答において中心的な役割を果たしているnuclear factor- k B(NF-k B)シグナリングに対するATRA の影響について,レポーター遺伝子解析法,リアルタイム定量的 RT-PCR法にて検討を行った.ATRA および他のレチノイド化合物(9-cis retinoic acid,13-cis retinoic acid,AM580,retinol)は,大腸癌由来細胞株において用量依存性に NF-k B を活性化した.また,ATRA はNF-k B の代表的な標的遺伝子であるIL-8の発現を誘導した.RAR の活性化と比較するとNF-k B 活性化に要するATRA の濃度域は高かった.TNF-a によるNF-k B の活性化,IL-8発現誘導は,ATRA をプレインキュベーションすることにより著明に増大し,相乗的な効果が認められた.ATRA はNF-k B サブユニット(RelA,p50)の発現を増大させ,TNF受容体(TNFR1)の発現レベルも上昇させていた.これらの結果より,レチノイン酸は大腸上皮細胞においてNF-k B シグナリングを活性化し,TNF-a に対する感受性を増大させている可能性が示唆された.", "subitem_description_type": "Other"}]}, "item_2_description_11": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "All-trans retinoic acid(ATRA)is a ligand for the retinoicacid receptor(RAR), a member of the nuclear receptorsuperfamily, and it is well established that RARs play acritical role in the development and differentiation of variousorgans including gastrointestinal tract. Recently, severalcase reports suggested a possible association between theclinical use of retinoic acid and inflammatory bowel diseases.However, it is not known whether ATRA affects the inflammatoryresponse of colonic epithelial cells. In this study,we examined the effect of ATRA on NF-k B activity andIL-8 expression in colonic epithelial cells in vitro. NF-k Bactivation and RAR activation were assessed by the reportergene assay and IL-8 mRNA expression was assessed bythe real-time quantitative RT-PCR. ATRA and other retinoidcompounds(9-cis retinoic acid, 13-cis retinoic acid,AM580 and retinol)activated NF-k B in a dose- and timedependentmanner in colonic cell lines(HCT116, Caco2,SW480, DLD-1, and LS174T). However, compared to RARactivation, much higher concentrations of ATRA wereneeded to activate NF-k B. ATRA also up-regulated theexpression of IL-8, a target gene of NF-k B. ATRA-inducedNF-k B activation was repressed by a MEK inhibitorand a p38 MAP kinase inhibitor. Preincubation with ATRAsignificantly potentiated TNF-a -induced activation of NFkB and TNF-a -induced expression of IL-8. ATRA wasfound to up-regulate the expression of NF-k B subunits(RelA and p50)and TNF-a receptor 1. These results suggestthat ATRA and other retinoid compounds can activateNF-k B signaling and potentiate the inflammatory responseto TNF-a in colonic epithelial cells.", "subitem_description_type": "Other"}]}, "item_2_description_15": {"attribute_name": "表示順", "attribute_value_mlt": [{"subitem_description": "5", "subitem_description_type": "Other"}]}, "item_2_description_16": {"attribute_name": "アクセション番号", "attribute_value_mlt": [{"subitem_description": "KJ00006372359", "subitem_description_type": "Other"}]}, "item_2_description_8": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_description": "原著", "subitem_description_type": "Other"}]}, "item_2_description_9": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_description": "Original", "subitem_description_type": "Other"}]}, "item_2_source_id_1": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00629581", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_19": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03855023", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_6": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "獨協医科大学内科学(消化器)"}, {"subitem_text_value": "獨協医科大学内科学(消化器)"}]}, "item_2_text_7": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Gastroenterology, Dokkyo Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Gastroenterology, Dokkyo Medical University"}]}, "item_2_title_3": {"attribute_name": "論文名よみ", "attribute_value_mlt": [{"subitem_title": "レチノインサン ワ ダイチョウ ジョウヒ サイボウ ニ オイテ NF-k B シグナリング オ カッセイカ スル"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "内園, まり子"}, {"creatorName": "ウチゾノ, マリコ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "3548", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "島田, 忠人"}, {"creatorName": "シマダ, タダヒト", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "3549", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Uchizono, Mariko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "3550", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimada, Tadahito", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "3551", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-05-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00006372359.pdf", "filesize": [{"value": "488.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 488100.0, "url": {"label": "KJ00006372359.pdf", "url": "https://dmu.repo.nii.ac.jp/record/827/files/KJ00006372359.pdf"}, "version_id": "7f483c88-4be5-4281-97ec-45e1f6af35d8"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "大腸上皮細胞", "subitem_subject_scheme": "Other"}, {"subitem_subject": "all-trans retinoic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NF-k B", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IL-8", "subitem_subject_scheme": "Other"}, {"subitem_subject": "TNF-a", "subitem_subject_scheme": "Other"}, {"subitem_subject": "colonic epithelial cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "all-trans retinoic acid", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NF-k B", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IL-8", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "TNF-a", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "レチノイン酸は大腸上皮細胞においてNF-k B シグナリングを活性化する", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "レチノイン酸は大腸上皮細胞においてNF-k B シグナリングを活性化する"}, {"subitem_title": "Retinoic acid activates NF-k B signaling in colonic epithelial cells", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "3", "path": ["53"], "permalink_uri": "https://dmu.repo.nii.ac.jp/records/827", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-05-24"}, "publish_date": "2017-05-24", "publish_status": "0", "recid": "827", "relation": {}, "relation_version_is_last": true, "title": ["レチノイン酸は大腸上皮細胞においてNF-k B シグナリングを活性化する"], "weko_shared_id": 3}