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Supplementation of Cryomedium with Catalase and N-acetylcysteine Improves Human Sperm Post-thaw Motility and DNA Integrity
https://dmu.repo.nii.ac.jp/records/1492
https://dmu.repo.nii.ac.jp/records/14929428c260-fb67-41cb-9af8-aefb4d275d53
名前 / ファイル | ライセンス | アクション |
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DJMS-44-2-Kobori-本文 (75.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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タイトル | ||||||
タイトル | Supplementation of Cryomedium with Catalase and N-acetylcysteine Improves Human Sperm Post-thaw Motility and DNA Integrity | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Antioxidant | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA damage | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sperm cryopreservation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kobori, Yoshitomo
× Kobori, Yoshitomo× Okada, Hiroshi× Niederberger, Craig |
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著者所属 | ||||||
Department of Urology, University of Illinois at Chicago:Department of Urology, Dokkyo Medical University Koshigaya Hospital | ||||||
著者所属 | ||||||
Department of Urology, Dokkyo Medical University Koshigaya Hospital | ||||||
著者所属 | ||||||
Department of Urology, University of Illinois at Chicago | ||||||
書誌情報 |
Dokkyo Journal of Medical Sciences 巻 44, 号 2, p. 135-140, 発行日 2017-07-25 |
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要旨 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Purpose:Reactive oxygen species have been suggested as a major contributing factor to cryodamage of the spermatozoa. Accordingly, antioxidant supplementation has been used to yield significantly improved quality of frozen sperm post-thaw. We sought to investigate outcomes with a combination of antioxidants catalase and N-acetylcysteine respectively. Methods:The cryomedium supplemented with 200U/ml catalase, 5 mM N-acetylcysteine and their combination was used. Semen samples were collected from normozoospermic men (n=20)and aliquots frozen in each dose of antioxidant tested. Post-thaw semen analysis by computer aided sperm analysis was performed. In addition, sperm viability (Eosin-Y staining) and sperm DNA fragmentation(TUNEL assay)were quantitated. Results:Combined catalase and N-acetylcysteine supplementation during cryopreservation resulted in significantly improved post-thaw recovery of total motility(from 39.4±15.8% to 49.2±16.1%) and percentage of sperm vitality (from 46.2±13.3% to 53.2±11.8%)(mean±SD, p<0.05). Catalase improved post-thaw DNA integrity analysis for doublestranded breaks using TUNEL assay. Conclusions:These results indicated that the combination of intracellular and extracellular antioxidants resulted in the pronounced effect in improving post-thaw quality of human spermatozoa. |
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記事種別 | ||||||
Original | ||||||
出版者 | ||||||
出版者 | 獨協医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855023 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00629581 | |||||
表示順 | ||||||
2 |