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Supplementation of Cryomedium with Catalase and N-acetylcysteine Improves Human Sperm Post-thaw Motility and DNA Integrity
https://dmu.repo.nii.ac.jp/records/1492
https://dmu.repo.nii.ac.jp/records/14929428c260-fb67-41cb-9af8-aefb4d275d53
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
DJMS-44-2-Kobori-本文 (75.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| タイトル | ||||||||||||
| タイトル | Supplementation of Cryomedium with Catalase and N-acetylcysteine Improves Human Sperm Post-thaw Motility and DNA Integrity | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Antioxidant | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | DNA damage | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Sperm cryopreservation | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
Kobori, Yoshitomo
× Kobori, Yoshitomo
× Okada, Hiroshi
× Niederberger, Craig
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| 著者所属 | ||||||||||||
| Department of Urology, University of Illinois at Chicago:Department of Urology, Dokkyo Medical University Koshigaya Hospital | ||||||||||||
| 著者所属 | ||||||||||||
| Department of Urology, Dokkyo Medical University Koshigaya Hospital | ||||||||||||
| 著者所属 | ||||||||||||
| Department of Urology, University of Illinois at Chicago | ||||||||||||
| 書誌情報 |
Dokkyo Journal of Medical Sciences 巻 44, 号 2, p. 135-140, 発行日 2017-07-25 |
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| 要旨 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | Purpose：Reactive oxygen species have been suggested as a major contributing factor to cryodamage of the spermatozoa. Accordingly, antioxidant supplementation has been used to yield significantly improved quality of frozen sperm post-thaw. We sought to investigate outcomes with a combination of antioxidants catalase and N-acetylcysteine respectively. Methods：The cryomedium supplemented with 200U/ml catalase, 5 mM N-acetylcysteine and their combination was used. Semen samples were collected from normozoospermic men （n＝20）and aliquots frozen in each dose of antioxidant tested. Post-thaw semen analysis by computer aided sperm analysis was performed. In addition, sperm viability （Eosin-Y staining） and sperm DNA fragmentation（TUNEL assay）were quantitated. Results：Combined catalase and N-acetylcysteine supplementation during cryopreservation resulted in significantly improved post-thaw recovery of total motility（from 39.4±15.8％ to 49.2±16.1％） and percentage of sperm vitality （from 46.2±13.3％ to 53.2±11.8％）（mean±SD, p＜0.05）. Catalase improved post-thaw DNA integrity analysis for doublestranded breaks using TUNEL assay. Conclusions：These results indicated that the combination of intracellular and extracellular antioxidants resulted in the pronounced effect in improving post-thaw quality of human spermatozoa. |
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| 記事種別 | ||||||||||||
| Original | ||||||||||||
| 出版者 | ||||||||||||
| 出版者 | 獨協医学会 | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 03855023 | |||||||||||
| 書誌レコードID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA00629581 | |||||||||||
| 表示順 | ||||||||||||
| 2 | ||||||||||||
