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Characterization of Urate Transport System in JAR and JEG-3 Cells, Human Trophoblast-derived Cell Lines
https://dmu.repo.nii.ac.jp/records/1475
https://dmu.repo.nii.ac.jp/records/147596755554-125b-4e72-b3b3-c733fc540677
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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タイトル | ||||||
タイトル | Characterization of Urate Transport System in JAR and JEG-3 Cells, Human Trophoblast-derived Cell Lines | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | urate | |||||
キーワード | ||||||
主題 | placenta | |||||
キーワード | ||||||
主題 | preeclampsia | |||||
キーワード | ||||||
主題 | hyperuricemia | |||||
キーワード | ||||||
主題 | transporters | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kiuchi, Kaori
× Kiuchi, Kaori× Morita, Asuka× Ouchi, Motoshi× Otani, Naoyuki× Jutabha, Promsuk× Nohara, Masakatsu× Fukasawa, Ichio× Fujita, Tomoe× Anzai, Naohiko |
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著者所属 | ||||||
Department of Pharmacology and Toxicology and Department of Obstetrics and Gynecology, Dokkyo Medical University School of Medicine | ||||||
著者所属 | ||||||
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine | ||||||
著者所属 | ||||||
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine | ||||||
著者所属 | ||||||
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine | ||||||
著者所属 | ||||||
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine | ||||||
著者所属 | ||||||
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine | ||||||
著者所属 | ||||||
Department of Obstetrics and Gynecology, Dokkyo Medical University School of Medicine | ||||||
著者所属 | ||||||
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine | ||||||
著者所属 | ||||||
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine:Department of Pharmacology, Chiba University Graduate School of Medicine | ||||||
書誌情報 |
Dokkyo Journal of Medical Sciences 巻 44, 号 1, p. 31-38, 発行日 2017-03-25 |
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要旨 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Urate (uric acid) is the major inert end product of purine metabolism in humans. Since it is water soluble, it requires a membranous protein called transporter for its permeation across the plasma membrane. Increased blood urate level is often seen in preeclampsia, but its precise mechanism remains unknown. Syncytiotrophoblasts function as a barrier between maternal blood and fetal one so called “blood-placental barrier”. So far, the expression of several urate transporters was identified in these cells, but it is still unclear about their contribution to urate handling in blood-placental barrier. In this study, we investigated the expression of urate transporters and the properties of [14C]urate transport in both JAR and JEG-3, human choriocarcinoma cells as a model of human placenta. Conventional PCR analysis revealed that both JAR and JEG-3 cells express strongly breast cancer resistance protein (BCRP/ABCG2) mRNA. Uptake of [14C]urate by these cells is time-dependent with Na+- and Cl--independent and voltage-insensitive manner and is not inhibited by benzbromarone, a representative renal urate transport inhibitor. Then, we focused on BCRP which shows strong mRNA expression and found that these cells have urate efflux property that is sensitive to fumitremorgin C (FMC), a BCRP inhibitor. These results suggest that BCRP is one of the important components for urate handling in human placenta in pathophysiological condition such as preeclampsia. | |||||
記事種別 | ||||||
Original | ||||||
出版者 | ||||||
出版者 | 獨協医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855023 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00629581 | |||||
表示順 | ||||||
6 |