{"_buckets": {"deposit": "53f0da16-d8e4-49e8-bd95-7750b1f2b9fc"}, "_deposit": {"created_by": 14, "id": "1475", "owners": [14], "pid": {"revision_id": 0, "type": "depid", "value": "1475"}, "status": "published"}, "_oai": {"id": "oai:dmu.repo.nii.ac.jp:00001475", "sets": ["86"]}, "author_link": ["6411", "6406", "6409", "6414", "6909", "6908", "6420", "1642", "6907"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-03-25", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "38", "bibliographicPageStart": "31", "bibliographicVolumeNumber": "44", "bibliographic_titles": [{"bibliographic_title": "Dokkyo Journal of Medical Sciences"}]}]}, "item_10001_description_34": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "Urate (uric acid) is the major inert end product of purine metabolism in humans. Since it is water soluble, it requires a membranous protein called transporter for its permeation across the plasma membrane. Increased blood urate level is often seen in preeclampsia, but its precise mechanism remains unknown. Syncytiotrophoblasts function as a barrier between maternal blood and fetal one so called “blood-placental barrier”. So far, the expression of several urate transporters was identified in these cells, but it is still unclear about their contribution to urate handling in blood-placental barrier. In this study, we investigated the expression of urate transporters and the properties of ［14C］urate transport in both JAR and JEG-3, human choriocarcinoma cells as a model of human placenta. Conventional PCR analysis revealed that both JAR and JEG-3 cells express strongly breast cancer resistance protein (BCRP/ABCG2) mRNA. Uptake of ［14C］urate by these cells is time-dependent with Na＋- and Cl－-independent and voltage-insensitive manner and is not inhibited by benzbromarone, a representative renal urate transport inhibitor. Then, we focused on BCRP which shows strong mRNA expression and found that these cells have urate efflux property that is sensitive to fumitremorgin C (FMC), a BCRP inhibitor. These results suggest that BCRP is one of the important components for urate handling in human placenta in pathophysiological condition such as preeclampsia.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "獨協医学会"}]}, "item_10001_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00629581", "subitem_source_identifier_type": "NCID"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03855023", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_text_24": {"attribute_name": "著者所属", "attribute_value_mlt": [{"subitem_text_value": "Department of Pharmacology and Toxicology and Department of Obstetrics and Gynecology, Dokkyo Medical University School of Medicine"}, {"subitem_text_value": "Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"}, {"subitem_text_value": "Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"}, {"subitem_text_value": "Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"}, {"subitem_text_value": "Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"}, {"subitem_text_value": "Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"}, {"subitem_text_value": "Department of Obstetrics and Gynecology, Dokkyo Medical University School of Medicine"}, {"subitem_text_value": "Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine"}, {"subitem_text_value": "Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine:Department of Pharmacology, Chiba University Graduate School of Medicine"}]}, "item_10001_text_28": {"attribute_name": "表示順", "attribute_value_mlt": [{"subitem_text_value": "6"}]}, "item_10001_text_33": {"attribute_name": "記事種別", "attribute_value_mlt": [{"subitem_text_value": "Original"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kiuchi, Kaori", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6907", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Morita, Asuka", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6420", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ouchi, Motoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6409", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Otani, Naoyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6406", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Jutabha, Promsuk", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6411", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nohara, Masakatsu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6908", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukasawa, Ichio", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1642", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujita, Tomoe", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6909", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Anzai, Naohiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6414", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-07-09"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "DJMS-44-1-6.pdf", "filesize": [{"value": "206.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 206200.0, "url": {"label": "DJMS-44-1-Kiuchi-本文", "url": "https://dmu.repo.nii.ac.jp/record/1475/files/DJMS-44-1-6.pdf"}, "version_id": "998c57ed-325d-4b10-83a4-0ab1c0fafbab"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "urate", "subitem_subject_scheme": "Other"}, {"subitem_subject": "placenta", "subitem_subject_scheme": "Other"}, {"subitem_subject": "preeclampsia", "subitem_subject_scheme": "Other"}, {"subitem_subject": "hyperuricemia", "subitem_subject_scheme": "Other"}, {"subitem_subject": "transporters", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Characterization of Urate Transport System in JAR and JEG-3 Cells, Human Trophoblast-derived Cell Lines", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Characterization of Urate Transport System in JAR and JEG-3 Cells, Human Trophoblast-derived Cell Lines"}]}, "item_type_id": "10001", "owner": "14", "path": ["86"], "permalink_uri": "https://dmu.repo.nii.ac.jp/records/1475", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-07-09"}, "publish_date": "2018-07-09", "publish_status": "0", "recid": "1475", "relation": {}, "relation_version_is_last": true, "title": ["Characterization of Urate Transport System in JAR and JEG-3 Cells, Human Trophoblast-derived Cell Lines"], "weko_shared_id": -1}