| Item type |
学術雑誌論文 / Journal Article(1) |
| タイトル |
|
|
タイトル |
Characterization of Urate Transport System in JAR and JEG-3 Cells, Human Trophoblast-derived Cell Lines |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
urate |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
placenta |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
preeclampsia |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
hyperuricemia |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
transporters |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kiuchi, Kaori
Morita, Asuka
Ouchi, Motoshi
Otani, Naoyuki
Jutabha, Promsuk
Nohara, Masakatsu
Fukasawa, Ichio
Fujita, Tomoe
Anzai, Naohiko
|
| 著者所属 |
|
|
|
Department of Pharmacology and Toxicology and Department of Obstetrics and Gynecology, Dokkyo Medical University School of Medicine |
| 著者所属 |
|
|
|
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine |
| 著者所属 |
|
|
|
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine |
| 著者所属 |
|
|
|
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine |
| 著者所属 |
|
|
|
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine |
| 著者所属 |
|
|
|
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine |
| 著者所属 |
|
|
|
Department of Obstetrics and Gynecology, Dokkyo Medical University School of Medicine |
| 著者所属 |
|
|
|
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine |
| 著者所属 |
|
|
|
Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine:Department of Pharmacology, Chiba University Graduate School of Medicine |
| 書誌情報 |
Dokkyo Journal of Medical Sciences
巻 44,
号 1,
p. 31-38,
発行日 2017-03-25
|
| 要旨 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Urate (uric acid) is the major inert end product of purine metabolism in humans. Since it is water soluble, it requires a membranous protein called transporter for its permeation across the plasma membrane. Increased blood urate level is often seen in preeclampsia, but its precise mechanism remains unknown. Syncytiotrophoblasts function as a barrier between maternal blood and fetal one so called “blood-placental barrier”. So far, the expression of several urate transporters was identified in these cells, but it is still unclear about their contribution to urate handling in blood-placental barrier. In this study, we investigated the expression of urate transporters and the properties of ［14C］urate transport in both JAR and JEG-3, human choriocarcinoma cells as a model of human placenta. Conventional PCR analysis revealed that both JAR and JEG-3 cells express strongly breast cancer resistance protein (BCRP/ABCG2) mRNA. Uptake of ［14C］urate by these cells is time-dependent with Na＋- and Cl－-independent and voltage-insensitive manner and is not inhibited by benzbromarone, a representative renal urate transport inhibitor. Then, we focused on BCRP which shows strong mRNA expression and found that these cells have urate efflux property that is sensitive to fumitremorgin C (FMC), a BCRP inhibitor. These results suggest that BCRP is one of the important components for urate handling in human placenta in pathophysiological condition such as preeclampsia. |
| 記事種別 |
|
|
|
Original |
| 出版者 |
|
|
出版者 |
獨協医学会 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03855023 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00629581 |
| 表示順 |
|
|
|
6 |
DJMS-44-1-Kiuchi-本文 (206.2 kB)
