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RING1A Regulates RING1B Expression and the Collapse of its Expression Impairs Neuroblastoma Cell Proliferation
https://dmu.repo.nii.ac.jp/records/2419
https://dmu.repo.nii.ac.jp/records/2419e8c35dbf-244b-43f3-8e0f-645aaf8cc230
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
DJMS-47-3-Hasegawa-本文 (1.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| タイトル | ||||||||||||
| タイトル | RING1A Regulates RING1B Expression and the Collapse of its Expression Impairs Neuroblastoma Cell Proliferation | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | RING1A | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | RING1B | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | neuroblastoma | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
Hasegawa, Mariko
× Hasegawa, Mariko
× Satoh, Shunpei
× Kamijo, Takehiko
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| 著者所属(英) | ||||||||||||
| en | ||||||||||||
| Department of Pediatric Surgery, Dokkyo Medical University Saitama Medical Center | ||||||||||||
| 著者所属(英) | ||||||||||||
| en | ||||||||||||
| Research Institute for Clinical Oncology, Saitama Cancer Center | ||||||||||||
| 著者所属(英) | ||||||||||||
| en | ||||||||||||
| Research Institute for Clinical Oncology, Saitama Cancer Center | ||||||||||||
| 書誌情報 |
Dokkyo Journal of Medical Sciences 巻 47, 号 3, p. 115-123, 発行日 2020-10-25 |
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| 要旨(英) | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | Neuroblastoma （NB） is the most common pediatric abdominal solid tumor with less than 50％ of longterm survival rate in high-risk cases. Ring finger protein 1/2, referred to as RING1A/B respectively, are E3 ubiquitin ligases that compose a catalytic subunit of Polycomb Repressive Complex-1 （PRC1）. PRC1 epigenetically down-regulates target gene transcription, especially tumor suppressor genes in tumorigenesis and cancer progression. However, the function of RING1 proteins is still unclear in NB. This study aims to explore the importance of RING1A （gene name, RING1） in human NB cells to evaluate its druggability. According to the Kaplan-Meier analysis based on the public NB patients’ transcript data, lower expression of RING1 showed poor prognosis. As such, we hypothesized the presence of the anti-tumorigenic function of RING1A. First, mouse Ring1A （mRing1A；gene name, Ring1） was transiently expressed in a NB cell line, NGP. The subsequent flat colony formation assay resulted in a decreased number of colonies, and intriguingly, endogenous RING1B expression was dampened in the transfectants. On top of that, we established NGP cells with inducible short hairpin （sh） RNA against RING1. Unexpectedly, short hairpin （sh） RING1 induction repressed cell proliferation. However, consistent with the transient expression of mRing1A, endogenous RING1B expression was elevated up on the shRING1 induction. Here, we showed that both gain and loss of RING1A expression suppressed NGP cell growth, and RING1A negatively controlled RING1B expression. Although the druggability of RING1A is still unclear, the current study suggests that RING1A does not functionally compensate RING1B, and the optimal expression of RING1A is essential for NB cell proliferation. |
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| 記事種別 | ||||||||||||
| Original | ||||||||||||
| 出版者 | ||||||||||||
| 出版者 | 獨協医学会 | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 03855023 | |||||||||||
| 書誌レコードID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA00629581 | |||||||||||
