| Item type |
学術雑誌論文 / Journal Article(1) |
| タイトル |
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タイトル |
Regulation of Glycerophospholipid and Cholesterol Synthesis in Cells by Hydroxylated Cholesterols (Oxysterols) |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
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主題Scheme |
Other |
|
主題 |
Oxysterols |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Phosphatidylethanolamine |
| キーワード |
|
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主題Scheme |
Other |
|
主題 |
Cholesterol |
| キーワード |
|
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主題Scheme |
Other |
|
主題 |
Pcyt2 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Hmgcr |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
25-Hydroxycholesterol |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Cytochrome P450 46A1 |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ando, Hiromi
Shinohara, Yasutake
Arai, Maki
Yamashita, Satoko
Sugimoto, Hiroyuki
|
| 著者所属 |
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Department of Biochemistry, Dokkyo Medical University Tochigi Japan |
| 著者所属 |
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Department of Biochemistry, Dokkyo Medical University Tochigi Japan |
| 著者所属 |
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Department of Biochemistry, Dokkyo Medical University Tochigi Japan |
| 著者所属 |
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Department of Biochemistry, Dokkyo Medical University Tochigi Japan |
| 著者所属 |
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Department of Biochemistry, Dokkyo Medical University Tochigi Japan |
| 書誌情報 |
Dokkyo Medical Journal
巻 1,
号 3,
p. 157-166,
発行日 2022-09
|
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Phosphatidylethanolamine (PE) and cholesterol are major lipid components of mammalian cell membranes. The rate limiting enzymes for the synthesis of PE and cholesterol are CTP: phosphoethanolamine cytidylyltransferase (Pcyt2) and 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), respectively. The transcription and activity of these enzymes in NIH3T3 cells are suppressed by fetal bovine serum (FBS) and hydroxylated cholesterol (oxysterols) in similar dose-dependent manners. These results suggested that the intrinsic oxysterol concentration in cells is important for maintaining membrane lipid components, leading us to investigate the regulation of oxysterol-producing enzyme activity in cells. Using NIH3T3 cells, we found that of all oxysterol-producing enzymes, only the 24S-hydroxycholesterol producing enzyme cytochrome P450 46A1 (Cyp46A1) is expressed. Transcription of Cyp46A1 is suppressed by FBS, and we identified insulin-like growth factor II (IGF-II) in FBS as the factor suppressing Cyp46A1 transcription. The oxysterol level in NIH3T3 cells is also suppressed by IGF-II. These results suggested that the classical hormone IGF-II regulates the oxysterol content in cells and transduces cellular signals. |
| 記事種別 |
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Review Article |
| 出版者 |
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出版者 |
Dokkyo Medical Society |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2436-522X |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.51040/dkmj.2022-018 |
DKMJ-1-3-Ando-Fulltext (970.0 kB)
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