| Item type |
学術雑誌論文 / Journal Article(1) |
| タイトル |
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タイトル |
N-Myc-activated microRNAs Inhibit Protein Synthesis of RUNX1 and RUNX3 in Neuroblastoma Cell Lines |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Neuroblastoma |
| キーワード |
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主題Scheme |
Other |
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主題 |
RUNX transcription factor |
| キーワード |
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主題Scheme |
Other |
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主題 |
N-Myc |
| キーワード |
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主題Scheme |
Other |
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主題 |
microRNA |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Streptavidin RNA aptamer |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Inoue, Ken-ichi
Akimoto, Kazumi
Inoue, Teruo
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| 著者所属 |
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Research Support Center, Dokkyo Medical University School of Medicine, Mibu, Japan |
| 著者所属 |
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Research Support Center, Dokkyo Medical University School of Medicine, Mibu, Japan |
| 著者所属 |
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Research Support Center, Department of Cardiovascular Medicine, Dokkyo Medical University School of Medicine, Mibu, Japan |
| 書誌情報 |
Dokkyo Journal Of Medical Sciences
巻 45,
号 2,
p. 43-51,
発行日 2018-07-25
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| 要旨 |
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内容記述タイプ |
Other |
|
内容記述 |
RUNX1 and RUNX3 are master transcription factors in sensory neuron lineage specifications. Protein levels of such developmental regulators are tightly controlled during carcinogenesis, in order to block differentiation and drive proliferation. Here we report that neuroblastoma specific microRNAs inhibit protein syntheses of RUNX1 and RUNX3 through 3’UTR sequences. Computational prediction identified two putative binding sequences for N-Myc-activated microRNAs both in RUNX1 and RUNX3 3’UTRs. Streptavidin RNA aptamer-tagged 3’UTR sequences pulled down miR-17, miR-18a, miR-19a, miR-20a or miR-130a from neuroblastoma cell lysate. 3’UTR target protection from N-Myc-activated microRNAs increased protein synthesis of RUNX1 or RUNX3 and induced differentiation in neuroblastoma cell lines. Together, protein levels of RUNX1 and RUNX3 are post-transcriptionally regulated by N-Myc-activated microRNAs, highlighting the mutual negative feedback between N-Myc oncogene and RUNX3 tumor suppressor in neuroblastoma. |
| 記事種別 |
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Original |
| 出版者 |
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出版者 |
獨協医学会 |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
03855023 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00629581 |
DJMS-45-2-Inoue-本文 (364.5 kB)
