| Item type |
学術雑誌論文 / Journal Article(1) |
| タイトル |
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タイトル |
Utility of a Single Particle Isolation System in Genome-editing and Gene-transfection Experiments |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Single particle isolation system |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Genome-editing |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Gene-transfection |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Single-cell cloning |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Sato-Yazawa, Hanako
Kashiwagi, Korehito
Ishii, Jun
Masawa, Meitetsu
Onozaki, Masato
Miyata-Hiramatsu, Chie
Miyazawa, Tadasuke
Sakaguchi, Mai
Yazawa, Takuya
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| 著者所属 |
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Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine |
| 著者所属 |
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Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine |
| 著者所属 |
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Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine |
| 著者所属 |
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Department of Respiratory Medicine, Dokkyo Medical University School of Medicine and Graduate School of Medicine |
| 著者所属 |
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Department of Diagnostic Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine |
| 著者所属 |
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Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine |
| 著者所属 |
|
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Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine |
| 著者所属 |
|
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Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine |
| 著者所属 |
|
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Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine |
| 書誌情報 |
Dokkyo Journal of Medical Sciences
巻 48,
号 2,
p. 75-80,
発行日 2021-07-25
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| 要旨 |
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内容記述タイプ |
Other |
|
内容記述 |
In recent decades, genetic engineering and its application to medical sciences has been rapidly progressing, and gene transfection and genome-editing techniques are generally used even in medical investigations. Although establishing single-cell clones are essential for confirming the cellular phenotypic changes in association with genetic modifications, it is technically hard to dispense single-cell efficiently and correctly. Here, we demonstrate single-cell cloning of genetically modified lung cancer cells using a single particle isolation system, On-chip SPiS, by which bi-allelic gene-knockout cells and highly gene-expressing transfectants can be easily and certainly established. In our experiment, we obtained one bi-allelic knockout clone from only 12 clones（8.3％）and eight highly transgene-expressing clones from 20 clones（40％）. Furthermore, we could define that considerable rate of clones without transgene were mixed among selection marker-resistant cell populations. These results suggest that the single-cell dispensing system is a quite useful tool for single-cell cloning in genome-editing and gene-transfection experiments. |
| 記事種別 |
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Original |
| 出版者 |
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出版者 |
獨協医学会 |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
03855023 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00629581 |
DJMS-48-2-Sato-Yazawa-本文 (238.4 kB)
