WEKO3
アイテム
Utility of a Single Particle Isolation System in Genome-editing and Gene-transfection Experiments
https://dmu.repo.nii.ac.jp/records/5007
https://dmu.repo.nii.ac.jp/records/500711e79b55-4ba1-4646-a852-6972151e8a0b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
DJMS-48-2-Sato-Yazawa-本文 (238.4 kB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| タイトル | ||||||
| タイトル | Utility of a Single Particle Isolation System in Genome-editing and Gene-transfection Experiments | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Single particle isolation system | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Genome-editing | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Gene-transfection | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Single-cell cloning | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Sato-Yazawa, Hanako
× Sato-Yazawa, Hanako× Kashiwagi, Korehito× Ishii, Jun× Masawa, Meitetsu× Onozaki, Masato× Miyata-Hiramatsu, Chie× Miyazawa, Tadasuke× Sakaguchi, Mai× Yazawa, Takuya |
|||||
| 著者所属 | ||||||
| 値 | Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Respiratory Medicine, Dokkyo Medical University School of Medicine and Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Diagnostic Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine | |||||
| 著者所属 | ||||||
| 値 | Department of Pathology, Dokkyo Medical University School of Medicine and Graduate School of Medicine | |||||
| 書誌情報 |
Dokkyo Journal of Medical Sciences 巻 48, 号 2, p. 75-80, 発行日 2021-07-25 |
|||||
| 要旨 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | In recent decades, genetic engineering and its application to medical sciences has been rapidly progressing, and gene transfection and genome-editing techniques are generally used even in medical investigations. Although establishing single-cell clones are essential for confirming the cellular phenotypic changes in association with genetic modifications, it is technically hard to dispense single-cell efficiently and correctly. Here, we demonstrate single-cell cloning of genetically modified lung cancer cells using a single particle isolation system, On-chip SPiS, by which bi-allelic gene-knockout cells and highly gene-expressing transfectants can be easily and certainly established. In our experiment, we obtained one bi-allelic knockout clone from only 12 clones（8.3％）and eight highly transgene-expressing clones from 20 clones（40％）. Furthermore, we could define that considerable rate of clones without transgene were mixed among selection marker-resistant cell populations. These results suggest that the single-cell dispensing system is a quite useful tool for single-cell cloning in genome-editing and gene-transfection experiments. | |||||
| 記事種別 | ||||||
| 値 | Original | |||||
| 出版者 | ||||||
| 出版者 | 獨協医学会 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03855023 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00629581 | |||||
